化合物ID-8 T2431

Product Introduction
Bioactivity


英文名: ID-8

描述: ID-8 是 DYRK 抑制剂，长时间培养能够维持胚胎干细胞的自我更新。

细胞实验: Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. (Only for Reference)

激酶实验: In Vitro Enzymatic Assay for Histone Deacetylases: In vitro activities of the 11 recombinant human zinc-dependent HDAC enzymes are detected by ?uorigenic release of 7-amino-4-methylcoumarin from substrate upon deacetylase enzymatic activity. A series of dilutions of the unique HDAC6 compound, tubacin, and SAHA are prepared with 10% DMSO in HDAC assay buffer, and 5 μL of the dilution was added to a 50-μL reaction so that the ?nal concentration of DMSO is 1% in all of the reactions. The enzymatic reactions are conducted in duplicate at 37 °C for 30 min in a 50-μL mixture containing HDAC assay buffer, 5 μg BSA, an HDAC substrate, an HDAC enzyme, and a test compound. After enzymatic reactions, 50 μL of 2× HDAC developer is added to each well, and the plate is incubated at room temperature for an additional 15 min. Fluorescence intensity is measured at an excitation of 360 nm and an emission of 460 nm using a Synergy microplate reader. Negative (no enzyme, no inhibitor, a drug with no HDAC inhibition activity) and positive controls (known HDAC inhibitor SAHA) are included in the assays. IC50 is determined at the drug concentration that results in 50% reduction of HDAC activity compared with the control.

体内活性: ID-8通过Sox2-Oct3 / 4激活维持Nanog基因表达,能够可逆地维持ESC细胞的长期培养。ID-8在Wnt存在的情况下,调节Wnt /β-连环蛋白信号,增加CBP /β-连环蛋白,来维持hESC的未分化状态,并维持细胞的增殖和存活。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 29.8 mg/mL (100 mM)


关键字: cell | embryonic | dual-specificity | DYRK | ESC | Inhibitor | inhibit | Dual specificity tyrosine phosphorylation regulated kinase | stem | regulated | phosphorylation | kinase | tyrosine | ID 8 | ID-8 | Dual specificity tyrosine regulated kinase

相关产品: HTH-01-091 | ML167 | ML 315 | AnnH31 | EHT 1610 | ProINDY | ARN25068 | DYRKs-IN-1 | Protein kinase inhibitors 1 | CK2/ERK8-IN-1

相关库: Anti-Aging Compound Library | Cell Cycle Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Kinase Inhibitor Library | Inhibitor Library | Tyrosine Kinase Inhibitor Library | Stem Cell Differentiation Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途