化合物KN-62 T2694

Product Introduction
Bioactivity


英文名: KN-62

描述: KN-62 是一种选择性的、可逆的钙调蛋白依赖性蛋白激酶 II (CaMK-II) 抑制剂，直接与 CaMK-II 酶的钙调蛋白结合位点结合，对大鼠脑 CaMK-II 的IC50值为 0.9 μM。它是非竞争性的P2X7受体拮抗剂，IC50约为 15 nM。

细胞实验: For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference)

激酶实验: Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid.

体外活性: KN-62作用于成年大鼠,降低其脑中癫痫诱导的脑源性神经生长因子mRNA的表达水平.

体内活性: KN-62抑制K562细胞的增殖,并阻断细胞周期进程。10 μM KN-62作用于大鼠胰岛细胞,抑制卡巴胆碱和钾刺激的胰岛素分泌。KN-62抑制PC12 D细胞中A23187诱导的Ca2+/CaM激酶磷酸化作用。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 72.2 mg/mL (100 mM)


关键字: noncompetitive antagonism | inhibit | KN62 | P2XRs | KN-62 | Calmodulin-dependent protein kinases | HEK293 cells | KN 62 | P2X Receptor | Calmodulin-dependent kinases | CaMK | Inhibitor | autophosphorylation

相关产品: Promethazine | CaM kinase II inhibitor TFA salt | Autocamtide-2-related inhibitory peptide | Y-33075 dihydrochloride | Autocamtide-2-related inhibitory peptide, myristoylated acetate(201422-04-0 free base) | Rimacalib | Autocamtide 2 TFA(129198-88-5 free base) | Rottlerin | Tanshinone IIA sulfonate sodium | CaMKIIα-PHOTAC

相关库: Ion Channel Inhibitor Library | Wnt/Hedgehog/Notch Compound Library | Bioactive Lipid Compound Library | Membrane Protein-targeted Compound Library | Neuronal Signaling Compound Library | Inhibitor Library | Kinase Inhibitor Library | Autophagy Compound Library | Covalent Inhibitor Library | Bioactive Compound Library

化合物KN-62 T2694信息由TargetMol中国为您提供，如您想了解更多关于化合物KN-62 T2694报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途