葛根素 T2815

Product Introduction
Bioactivity


英文名: Puerarin

描述: Puerarin (Kakonein) 是从葛根中提取的一种异黄酮，是一种5-HT2C 受体拮抗剂。

细胞实验: RAW264.7 cells are maintained at subconfluence in 95% air and 5% CO2 humidified atmosphere maintained at 37°C. The medium used for routine subculture is Dulbecco's Modified Eagle's Medium supplemented with 10% fetal bovine serum, penicillin (100 units/mL) and streptomycin (100 μg/ mL). An MTT assay is used to measure the viability of the cells after treatment with puerarin. After the supernatants are removed for nitrite determination, cells are incubated at 37°C with MTT (0.05 mg/mL) for 4 h, and the optical density is measured at 540 nm. The concentrations of puerarin are10, 20, 40 and 100 μM[1].

激酶实验: STAT3-dependent dual-luciferase assay: HCT-116 cells are transiently transfected with reporter plasmid having the STAT3-binding element for regulating luciferase assay. Cells are treated with Cryptotanshinone for 24 hours at a concentration range of 0.2 to 50 μM. After treatment, cells are harvested in 20 μL of passive lysis buffer and luciferase activity is evaluated by the Dual Luciferase Reporter Assay kit on Wallac Victor2. The concentration of Cryptotanshinone that inhibits the luciferase activity by 50% represents IC50 value.

体外活性: 给高胆固醇饮食的大鼠每天口服300 mg/kg Puerarin,显著降低高胆固醇饮食引起的血清和肝脏中总胆固醇含量的增高.

体内活性: Puerarin在25 μM下剂量依赖性减少HT-29细胞的生长,伴随bax的增加,以及c-myc和bcl-2的减少。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (120.08 mM)
5% DMSO+95% Saline : 4 mg/mL (9.61 mM, suspension)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
H2O : < 1 mg/mL (insoluble or slightly soluble)


关键字: 5-HT Receptor | 5-hydroxytryptamine Receptor | inhibit | Puerarin | Serotonin Receptor | Inhibitor

相关产品: Metoclopramide hydrochloride hydrate | 8-Hydroxy-DPAT hydrobromide | 5-HT6R antagonist 1 | NPS ALX Compound 4a | PU02 | Talipexole dihydrochloride | Cyclobenzaprine hydrochloride | 2-Methyl-5-HT maleate | Naratriptan | Dehydroaripiprazole

相关库: Drug Repurposing Compound Library | Anti-Neurodegenerative Disease Compound Library | Anti-Cancer Drug Library | Membrane Protein-targeted Compound Library | Inhibitor Library | GPCR Compound Library | Anti-Cancer Clinical Compound Library | Anti-Cancer Approved Drug Library | Traditional Chinese Medicine Monomer Library | Tibetan medicine Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途