丹参酮IIA T2906

Product Introduction
Bioactivity


英文名: Tanshinone IIA

描述: Tanshinone IIA (Tanshinone B) 是红根丹参根系中的一种主要组合物。它能够利用靶向 VEGF/VEGFR2的蛋白激酶结构域，抑制血管生成。

细胞实验: Tanshinone IIA (Tan IIA) is dissolved in DMSO (10 mM) and stored (in dark and -20°C), and serially diluted in a RPMI 1640 medium immediately prior to experiments[1]. A549 cells are counted in logarithmic phase and 6000 cells (90 μL volume) are placed in 96-well plates. 10 μL varying concentrations of Tanshinone IIA (final concentrations 80, 60, 40, 30, 20, 15, 10, 5 and 2.5 μM) and ADM (final concentrations 8, 4, 2, 1, 0.5 and 0.25 μM) are added into drug groups, while negative control group (vehicle group) is only added 10 μL DMSO or normal saline without Tanshinone IIA or ADM. Cells are incubated for an additional 2 h with CCK-8 reagent (100 μL/mL medium) and the absorbance is read at 450 nm using a microplate reader. Cell proliferation inhibition rates are calculated according to the following formula: the proliferation inhibition ratio (%)=1-[(A1-A4)/(A2-A3)]×100, where, A1 is the OD value of drug experimental group, A2 is the OD value of blank control group, A3 is the OD value of the RPMI1640 medium without cells, and A4 is the OD value of drugs with the same concentration as A1 but without cells. The IC50 value, which represents the concentration of the drug that demonstrates 50% of cell growth inhibition, is calculated by nonlinear regression analysis using GraphPad Prism software[1].

体外活性: 在大鼠体内,灌胃Tanshinone IIA的LD50为25 g/kg.

体内活性: 在心肌细胞中,Tanshinone IIA能够抑制氧化应激诱导的细胞凋亡。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 2.94 mg/mL (10 mM)


关键字: inhibit | VEGFR | Vascular endothelial growth factor receptor | Tanshinone IIA | Inhibitor

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