化合物MS049 2HCl T4393

Product Introduction
Bioactivity


英文名: MS049 2HCl (1502816-23-0(free base))

描述: MS049 2HCl (1502816-23-0(free base)) 是一种有效的选择性 PRMT4 (IC50 = 34 nM) 和 PRMT6 (IC50 = 43 nM) 抑制剂。它对其他 I 型 PRMT 的活性较低（对于 PRMT1、PRMT3 和 PRMT8，IC50分别>130、>220 和 1.6 μM，），并且对 II 型或 III 型 PRMT 没有抑制作用，也没有对任何其他甲基转移酶或非表观遗传靶标进行测试。

细胞实验: HEK293 cells were grown in 12-well plates in DMEM supplemented with 10% FBS, penicillin (100 units/mL) and streptomycin (100 μg/mL). 30% confluent cells were treated with different concentrations of compounds in triplicates or DMSO control for 72 h. Cells were lysed in 100 μL of total lysis buffer (20 mM Tris-HCl pH 8, 150 mM NaCl, 1 mM EDTA, 10 mM MgCl2, 0.5% TritonX-100, 12.5 U/mL benzonase, complete EDTA-free protease inhibitor cocktail ). After 3 min incubation at RT, SDS was added to final 1% concentration. Lysates were run on SDS-PAGE and immunoblotting was done as outlined below to determine Med12-Rme2a levels in Western blot. They are for reference only.

体外活性: MS049 has been shown to reduce the H3R2me2a mark in HEK293 cells with an IC50 value of 0.97 μM and also, unexpectedly, to reduce H4R3me2a in HEK293 cells.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: PBS(pH7.2) : 10 mg/ml
DMSO : 30 mg/mL
Ethanol : 30 mg/mL


关键字: MS049 2HCl (1502816230(free base)) | MS049 2HCl (1502816 23 0(free base)) | MS049 2HCl (1502816-23-0(free base)) | MS-049 2HCl (1502816-23-0(free base))

相关产品: Tazemetostat hydrobromide | MM-102 TFA | Valemetostat | MS049 | TC-E 5003 | MI-538 | DC-05 | Amodiaquine dihydrochloride dihydrate | MR837 | MS37452

相关库: Methylation Compound Library | Reprogramming Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途