硝呋替莫 T5325

Product Introduction
Bioactivity


英文名: Nifurtimox

描述: Nifurtimox (BAY-A-2502) 是一种用于锥虫病的抗虫剂，可影响乳酸脱氢酶活性。它有潜力研究神经母细胞瘤细胞。

细胞实验: To assess the cell viability after incubation with nifurtimox at different concentrations (10 μg/mL up to 50 μg/mL or 34.8 μM to 174 μM, respectively in the supernatant growth medium) or the vehicle control with according concentrations, all neuroblastoma cell lines were subjected to an MTS assay. Stock solutions of MTS were made at 480 μM in sterile filtered deionized water and stored at ?20°C. Cells were grown to approximately 50% confluency, treated with nifurtimox, and incubated for 1 h with fresh media containing 12 μM MTS. The supernatant was subsequently removed and the cells were lysed with DMSO containing 10% (w/v) sodium dodecyl sulfate (SDS) and 1% (v/v) glacial acetic acid (Carl Roth, #3738). Purple formazan contents of each cell lysate were photometrically analyzed in triplicates at 570 nm (630 nm reference wavelength) in 96 microtiter plates [2].

体外活性: Nifurtimox can produce anion radicals and interfere with oxygen metabolism [1]. On the neuroblastoma cell lines LA-N-1, IMR-32, LS and SK-N-SH, the treatment of nifurtimox shows an increased production of oxidative stress, a reduced lactate dehydrogenase enzyme activity and reduced lactate production. Furthermore, nifurtimox leads to reduced mRNA and protein levels of the proto-oncogene protein N-Myc [2].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : Insoluble
DMSO : 55 mg/mL (191.44 mM)


关键字: LDH | Lactate Dehydrogenase | Parasite | BAY2502 | BAY-A 2502 | inhibit | BAY 2502 | Nifurtimox | Inhibitor | BAY-A2502

相关产品: hDHODH-IN-8 | CPS-11 | Enasidenib | DSM265 | Mutant IDH1 inhibitor | Mutant IDH1-IN-1 | Kakkalide | 3-Hydroxybenzaldehyde | Tenovin-6 Hydrochloride | CHIKV-IN-2

相关库: FDA-Approved Drug Library | Drug Repurposing Compound Library | Anti-Cancer Drug Library | Clinical Compound Library | Anti-Cancer Clinical Compound Library | Anti-Parasitic Compound Library | Anti-Cancer Approved Drug Library | Anti-Cancer Active Compound Library | Anti-Infection Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途