化合物UAMC-3203 T5343

Product Introduction
Bioactivity


英文名: UAMC-3203

描述: UAMC-3203 是一种有效的选择性铁死亡抑制剂，IC50为12 nM。

细胞实验: In order to determine IC50-values, human neuroblastoma cells (IMR-32) were seeded in a 96-well plate at a density of 25,000 cells/well. The next day, the cells were pretreated for 1h (in triplicates) with a 1/3 dilution series of ferrostatin-1 analogues ranging from 5μM to 0.68nM and Sytox Green (1.6 μM) and a 1/2 dilution series of ferrostatin-1 analogues ranging from 200 nM to 0.78nM and Sytox Green (1.6 μM) for erastin and ferrous ammonium sulphate respectively. After stimulating the cells with erastin (10 μM) or ferrous ammonium sulphate (600 μM), the plate was transferred to a temperature- and CO2-controled FLUOstar Omega fluorescence plate reader. Sytox Green intensity was measured after 13h using an excitation filter of 485 nm and an emission filter of 520 nm. In each setup, Triton-X100 (0.05%) was used to induce lyses of the cells in 6 wells/plate, and was used as 100% cell death reference. The percentage of the cell death was calculated by the formula ((AVG[erastin] - AVG[background]) /(AVG[Triton-X100] - AVG[background])) × 100. Cell death percentage was plotted in GraphPad Prism 6, and IC50-values were calculated using a sigmoidal dose-response (variable slope) curve.

动物实验: Vehicle solution (2% DMSO) or compound was administered daily at a concentration of 2 mM (in 0.9% NaCl containing 2% DMSO; 200 μL / 20 g body weight) by intraperitoneal injection. Body temperature and weight were monitored daily. On day 28 the mice were anesthetized with isoflurane and blood was sampled. Hereafter, mice were sacrificed by cervical dislocation. ALT, AST and LDH levels in plasma were measured. CK, creatinine, urea and troponin T were determined.

体外活性: UAMC-3203 exhibits even lower IC50-values (10 nM in IMR32 Neuroblastoma cells.) combined with an excellent kinetic solubility (> 200 μM).

体内活性: In blood plasma of mice having received a daily injection of compound UAMC-3203 over a period of 4 weeks, Plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), LDH, creatine kinase (CK), creatinine, urea and troponin T levels were all comparable to background values detected in control mice.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (106.01 mM)


关键字: inhibit | Ferroptosis | Inhibitor | UAMC-3203

相关产品: D-glutamine | Deferasirox | Pifithrin-β hydrobromide | Ferrostatin-1 | (-)-Epicatechin | Simvastatin | SRS11-92 | Imidazole ketone erastin | L-Glutamic acid | NCOA4 - 9a

相关库: Anti-COVID-19 Compound Library | Cell Cycle Compound Library | Bioactive Compounds Library Max | Ferroptosis Compound Library | Inhibitor Library | Apoptosis Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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