化合物JG-98 T5350
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mg | 1456551-16-8 | ¥1,080.00 | 询底价 |
10 mg | 1456551-16-8 | ¥3,960.00 | 询底价 |
100 mg | 1456551-16-8 | ¥11,900.00 | 询底价 |
5 mg | 1456551-16-8 | ¥2,530.00 | 询底价 |
25 mg | 1456551-16-8 | ¥6,480.00 | 询底价 |
2 mg | 1456551-16-8 | ¥1,660.00 | 询底价 |
50 mg | 1456551-16-8 | ¥8,850.00 | 询底价 |
Product Introduction
Bioactivity
英文名: JG-98
描述: JG-98 是一种热休克蛋白 70 (Hsp70)变构抑制剂,可与 Hsp70 上的保守位点紧密结合,打断 Hsp70-Bag3 相互作用。它影响癌细胞和肿瘤相关巨噬细胞,具有抗癌活性。
细胞实验: Cell viability was determined using an MTT colorimetric assay with the following modifications. Briefly, cells (5 x 10^3 ) were plated into 96-well assay plates in 0.1 ml media and allowed to attach overnight. Cells were then treated with compound at various concentrations in 0.2 mL media. After the 72-hour incubation period, cells were washed in PBS (3 x 100 μL), and 10 μL MTT reagent was added with 100 μL fresh media. Cells were then incubated for 4 hr in a humidified chamber at 37 oC with 5% CO2. Insoluble formazan crystals were solubilized by addition of 0.1 mL detergent solution (4 hr at room temp., dark). Resulting colored solutions were then quantified at an absorbance of 570 nm [1].
动物实验: Briefly, one million MCF7 or HeLa cells in Matrigel were subcutaneously injected bilaterally into 6 week old NCR mice. Once tumors were established, JG-98 (3 mg/kg; n=5) or vehicle control (1:1 PBS: DMSO; n=5) was introduced interperitoneally on days 2, 4 and 6. Tumor growth (10 tumors/5 mice) was measured by caliper every other day [2].
体外活性: JG-98 had a potency of 0.4 ± 0.03 μM against MDA-MB-231 cells and an EC50 value of 0.7 ± 0.2 μM for MCF7 cells. In MDA-MB-231 cells, the treatment of JG-98 activated apoptotic mediators (caspase-3 and PARP). Treatment of both MDA-MB-231 and MCF7 cells with JG-98 strongly affected autophagic flux [1]. JG-98 has antiproliferative activity (EC50 values between 0.3 and 4 μmol/L) across cancer cell lines from multiple origins. JG-98 destabilized FoxM1 and relieved suppression of downstream effectors, including p21 and p27 [2].
体内活性: JG-98(3 mg/kg)在HeLa异种移植模型中抑制了肿瘤生长,尽管效果较弱[2]。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 5 mg/mL (9.35 mM)
关键字: HSP | Apoptosis | JG-98 | inhibit | Heat shock proteins | Inhibitor
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途