1-氨基苯并三唑 T5953

Product Introduction
Bioactivity


英文名: 1-Aminobenzotriazole

描述: 1-Aminobenzotriazole (ABT) 是一种不可逆的细胞色素 P450 非特异性抑制剂。

细胞实验: Animals were killed by cervical dislocation and decapitation.?Kidneys and livers were excised quickly and placed in ice cold 1.15% KC].?Renal inner medulla and papilla were discarded.?Renal cortex and liver were minced and homogenized in 3 vol of 100 mM phosphate buffer (pH 7.4) containing 250 mM sucrose and 1.5 mM EDTA.?Kidney and liver homogenates were centrifuged at 10000g for 20 min. The resulting supematant was centrifuged at 105000g for 60 min. The 105000g supematant (cytosol) was used for the determination of glutathione S-transferase activity .?The microsomal fraction (pellet) was resuspended in phosphate buffered sucrose (pH 7.4) and centrifuged at 105000g for 60 min. The resulting microsomal fraction was resuspended in phosphate-buffered sucrose (pH 7.4) containing 20% glycerol to a final concentration of l0-20 mg protein per milliliter.?The microsomal fraction was used for the determination of P450, cytochrome b5, and NADPH-cytochrome-c reductase activities.?Microsomes from control or ABT-treated rats were either analyzed on the day of preparation or stored overnight at -80 C. In all cases, control microsomes were handled identically with ABT-treated microsomes.?P450 content in control and ABT-treated microsomes was not affected by overnight storage[1].

动物实验: For in vivo experiments, rats received ABT (O-100 mg/kg) dissolved in normal saline at a concentration of I-50 mg/ml.?ABT was administered ip and rats were killed at various times thereafter.?Total injection volume was either 1 or 2 ml/kg and control rats received 2 ml saline/kg[1].

体外活性: In vitro, ABT rapidly and efficiently destroyed P450 in both hepatic and renal microsomes prepared from naive male Sprague-Dawley rats.?Incubation of hepatic or renal microsomes in vitro with ABT produced detectable destruction of P450 within 5 min. Maximal destruction of P450 occurred within 10 min in both hepatic and renal microsomes during in vitro incubation with ABT. ABT-induced destruction of P450 in vitro was concentration-dependent.?For hepatic microsomes, maximal destruction of about 70% of P450 required concentrations of ABT equal to or greater than 10 mM. For renal microsomes, maximal destruction of about 80% of P450 required concentrations of ABT equal to or greater than 10 mM. In both liver and kidney, only P450 content and P450-dependent activities were significantly decreased[1].

体内活性: Hepatic and renal microsomes and cytosol were prepared from male Sprague-Dawley rats following ABT pretreatment (0-100 mg/kg ip) for various times.?Administration of 100 mg ABT/kg produced profound reductions in P450 content in both liver and kidney within 2 hr;?loss of P450 in both tissues persisted for at least 48 hours.?ABT-induced destruction of P450 was dose-dependent.?Maximal destruction of about 80% of total hepatic P450 occurred at dosages of ABT equal to or greater than 10 mg/kg.?Maximal destruction of about 80% of total renal P450 occurred at dosages of ABT equal to or greater than 50 mg/kg[1].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 125 mg/mL (931.86 mM)


关键字: inhibit | Cytochrome P450 | 1-Aminobenzotriazole | 1Aminobenzotriazole | 1 Aminobenzotriazole | Inhibitor | CYPs

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相关库: Anti-Aging Compound Library | Bioactive Compounds Library Max | Anti-Metabolism Disease Compound Library | Human Metabolite Library | Inhibitor Library | Lipid Metabolism Compound Library | Metabolism Compound Library | Covalent Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library

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