化合物MM-102 T6333
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
2 mg | 1417329-24-8 | ¥622.00 | 询底价 |
10 mg | 1417329-24-8 | ¥1,980.00 | 询底价 |
50 mg | 1417329-24-8 | ¥7,360.00 | 询底价 |
100 mg | 1417329-24-8 | ¥9,930.00 | 询底价 |
500 mg | 1417329-24-8 | ¥19,900.00 | 询底价 |
1 mg | 1417329-24-8 | ¥428.00 | 询底价 |
5 mg | 1417329-24-8 | ¥1,280.00 | 询底价 |
1 mL | 1417329-24-8 | ¥1,790.00 | 询底价 |
25 mg | 1417329-24-8 | ¥4,230.00 | 询底价 |
Product Introduction
Bioactivity
英文名: MM-102
描述: MM-102 (HMTase Inhibitor IX) 是一种有效的 WDR5/MLL 相互作用抑制剂,在 WDR5 结合检测中,IC50为 2.4 nM,Ki 小于 1 nM。
细胞实验: MV4;11, KOPN8, and K562 cells are cultured in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum and 100 U/L penicillin-streptomycin and incubated at 37 °C under 5% CO2. Cells are seeded into 12-well plates for suspension at a density of 5 × 105 per well (1 mL) and treated with either vehicle control (DMSO, 0.2%) or MM-102 for 7 days. The medium is changed every 2 days, and compounds are resupplied. The CellTiter-Glo Luminescent Cell Viability Assay kit is used following the manufacturer’s instruction. First, 100 μL of the assay reagent is added into each well, and the content is mixed for 2 min on an orbital shaker to induce cell lysis. After 10 min incubation at room temperature, the luminescence is read on a microplate reader. (Only for Reference)
激酶实验: In Vitro Histone Methyltransferase (HMT) Assay: The HMT assay is performed in 50 mM HEPES pH 7.8, 100 mM NaCl, 1.0 mM EDTA, and 5% glycerol at 22 °C. Each reaction contains 1.5 μCi of the co-factor, 3H-S-adenosylmethionine. H3 10-residue peptide is used as the substrate at 50 μM. Compounds are added at concentrations ranging from 0.125 to 128 μM and incubated with the pre-assembled WDR5/RbBP5/ASH2L complex at a final concentration of 0.5 μM for each protein for 2–5 min. Reactions are initiated by addition of the MLL1 protein at a final concentration of 0.5 μM and allowed to proceed for 30 min before preparing scintillation counting. To count samples, reactions are spotted on separate squares of P81 filter paper and precipitated by submerging in freshly prepared 50 mM sodium bicarbonate buffer with pH 9.0. After washing and drying, samples are vortexed in Ultima Gold scintillation fluid and counted. As a negative control, assays are performed using 0.5 μM MLL1/WDR5/RbBP5/ASH2L complex assembled with the non-interacting mutant, WDR5D107A.
体外活性: MM-102, as a MLL1 mimetic, shows high binding affinities to WDR5 with IC50 of 2.9 nM and Ki of < 1 nM. In the MLL1-AF9 transduced murine cells, MM-102 specifically reduces expression of two critical MLL1 target genes (HoxA9 and Meis-1), which are required for MLL1 mediated leukemogenesis. In addition, MM-102 effectively and selectively inhibits cell growth and induces apoptosis in leukemia cells harboring MLL1 fusion proteins. [1]
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 93 mg/mL (138.8 mM)
H2O : 92 mg/mL (137.4 mM)
Ethanol : 93 mg/mL (138.8 mM)
关键字: MM 102 | Inhibitor | MM-102 | inhibit | Histone Methyltransferase
相关产品: C-7280948 | EZM0414 | PRMT5-IN-28 | MM-102 TFA | UNC1215 | Antitumor agent-101 | A-366 | M‑89 | WDR5-0102 | UNC6852
相关库: Bioactive Compounds Library Max | Inhibitor Library | Epigenetics Compound Library | PPI Inhibitor Library | Histone Modification Compound Library | Bioactive Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途