N-[(4-甲基苯基)磺酰基]-L-精氨酸甲酯 T6693

Product Introduction
Bioactivity


英文名: TAME

描述: TAME (N-4-Tosyl-L-arginine methyl ester) 是后期促进复合体(APC/C 或 APC)的抑制剂，可与 APC/C 结合并阻止其通过 Cdc20 和 Cdh1 活化，产生有丝分裂阻滞。

体外活性: TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 μM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC. [1] TAME is hydrolysed by trypsin with Km of 0.328 mM. [2] TAME accelerates the ATP hydrolysis process about 12-fold. [3] TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring. [4] TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis. [5] TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M. [6]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 63 mg/mL (184 mM)
Ethanol : 3 mg/mL (8.76 mM)
DMSO : 64 mg/mL (186.9 mM)


关键字: TAME | Inhibitor | Anaphase promoting complex | APC | inhibit

相关产品: CFM 4 | TASIN-1 | TASIN-1 Hydrochloride | TAME hydrochloride | Apcin | Activated Protein C (390-404), human acetate | Apcin A HCL

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途