3,4-二羟基苯甲羟肟酸 T7525

Product Introduction
Bioactivity


英文名: Didox

描述: Didox (NSC-324360) 是一种合成的核糖核苷酸还原酶抑制剂，可降低患有痴呆症的 HIV 患者大脑中氧化损伤标志物的水平。

细胞实验: The cells were seeded in a 96-well plate (at a density of 2 × 10^3 cells for HA22T/VGH;?1.5 × 103 cells for HuH7) and exposed to various concentrations of didox and only HA22T/VGH also to hydroxyurea, DFO or DFP (0, 1, 10, 25, 50, 100, 200 and 500 μM) for 24, 48 and 72 h. In other experiments, HA22T/VGH were seeded in 96-well plates and treated with a single dose of didox, HU, DFO, DFP alone or in combination with increasing doses of FAC (25, 50, 100, 200 and 400 μM) for 48–72 h. In other type of treatment,?HA22T/VGH cells were or pre-treated for 16 h with a single dose of didox (200 μM) and then treated in combination with FAC (400–800 μM) or directly in combination didox-FAC for 48–72 h.Cell viability was evaluated with an MTT assay.?After the indicated time points and treatments, the supernatant was removed and 100 μL of the MTT solution (0.5 mg/mL) diluted in the cell medium was added to the wells.?After 3.5 h of incubation at 37 °C and 5% CO2, the MTT medium was removed and 75 μL of DMSO was added to each well.?Plates were shaken for 15 min at 37 °C until complete dissolution and absorbance was measured at 540 nm emission wavelengths.?Average percentage of cell viability at each concentration was calculated using Microsoft Excel 2016 software[1].

体外活性: Didox induced cell death and that this effect was suppressed by iron supplementation.?Cell treatments with didox caused changes of cellular iron content, TfR1 and ferritin levels comparable to those caused by the iron chelators, deferoxamine (DFO) and deferiprone (DFP).Didox is a bidentated iron chelator with two theoretical possible positions for the binding and among them that with the two hydroxyls of the catechol group acting as ligands is the more likely one. The iron chelating property of didox may contribute to its antitumor activity not only blocking the formation of the tyrosil radical on Tyr122 (such as HU) on RRM2 (essential for its activity) but also sequestering the iron needed by this enzyme and to the cell proliferation[1].

体内活性: Didox处理小鼠源骨髓诱发的肥大细胞(BMMC)时，降低了IgE刺激下的脱颗粒反应和包括IL-6、IL-13、TNF及MIP-1a (CCL3)在内的细胞因子产生[2]。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 100 mg/mL (591.26 mM)


关键字: Didox | NSC 324360 | Inhibitor | DNA/RNA Synthesis | inhibit | NSC324360

相关产品: Fidaxomicin | Rifabutin | 2'-Deoxyuridine | Vidofludimus | Procaine hydrochloride | N2-methylguanine | Flucytosine | Kobophenol A | COH29 | Pyridostatin

相关库: Cell Cycle Compound Library | ReFRAME Related Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Human Metabolite Library | Inhibitor Library | Preclinical Compound Library | Anti-Viral Compound Library | Anti-Infection Compound Library | Bioactive Compound Library

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