盐酸伊立替康三水合物 T0486
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
200 mg | 136572-09-3 | ¥1,280.00 | 询底价 |
10 mg | 136572-09-3 | ¥233.00 | 询底价 |
50 mg | 136572-09-3 | ¥538.00 | 询底价 |
500 mg | 136572-09-3 | ¥2,620.00 | 询底价 |
25 mg | 136572-09-3 | ¥372.00 | 询底价 |
100 mg | 136572-09-3 | ¥800.00 | 询底价 |
1 mL | 136572-09-3 | ¥582.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Irinotecan hydrochloride trihydrate
描述: Irinotecan hydrochloride trihydrate (CPT-11 HCl Trihydrate) 通过抑制拓扑异构酶 1 来防止 DNA 展开,可研究结肠癌和直肠癌。
细胞实验: Exponentially growing cells (LoVo and HT-29 cells) are seeded in 20 cm2 Petri dishes with an optimal cell number for each cell line (2 × 104 for LoVo cells, 105 for HT-29 cells). They are treated 2 days later with increasing concentrations of Irinotecan or SN-38 for one cell doubling time (24 hours for LoVo cells, 40 hours for HT-29 cells). After washing with 0.15 M NaCl, the cells are further grown for two doubling times in normal medium, detached from the support with trypsin-EDTA and counted in a hemocytometer. The IC50 values are then estimated as the Irinotecan or SN-38 concentrations responsible for 50% growth inhibition as compared with cells incubated without Irinotecan or SN-38. (Only for Reference)
体外活性: 在肝脏,胃,十二指肠和结肠中,单剂量的Irinotecan能够显著增加拓扑异构酶I与DNA的共价结合数量.并且与对照组相比,Irinotecan处理组的结肠黏膜细胞中DNA链断裂数量明显较高.在COLO320异种移植物中,Irinotecan诱导92%的最大生长抑制.
体内活性: 在LoVo细胞和HT-29细胞系中,IC50浓度的Irinotecan诱导相似数量的可裂解复合物。SN-38浓度依赖性的诱导可裂解复合物的形成,这在LoVo细胞和HT-29细胞系中没有明显差异。Irinotecan在细胞中的积聚存在显著差异,在HT-29细胞中的水平始终高于LoVo细胞。 在血浆,肠道和肿瘤组织中,Irinotecan被羧酸酯酶活化为SN-38(主要经肝脏活化),从而可与其靶点,拓扑异构酶I相互作用。在水溶液中Irinotecan的内酯E环和SN-38水解是可逆的,其羧酸盐和内酯形式的互变取决于温度和PH。在肿瘤和正常组织中,对于同一浓度的SN-38葡糖苷酸和Irinotecan,β-葡糖醛酸酶介导的SN-38产率均高于Irinotecan 形成的SN-38产率。在SCLC细胞系中,Irinotecan的活性明显高于NSCLC细胞系,而在组织学分型中并未观察到SN-38有明显的差异。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: H2O : 1 mg/mL (1.47 mM)
Ethanol : 7 mg/mL (10.33 mM)
DMSO : 45 mg/mL (66.45 mM)
关键字: CPT-11 hydrochloride | inhibit | Irinotecan hydrochloride | Inhibitor | (+)-Irinotecan hydrochloride | Topoisomerase | Irinotecan hydrochloride trihydrate | Irinotecan hydrochloride Trihydrate | Irinotecan HCl | Irinotecan Hydrochloride | Autophagy | CPT-11 HCl
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相关库: Anti-Aging Compound Library | Drug Repurposing Compound Library | Anti-Cancer Drug Library | Anti-Cancer Clinical Compound Library | Inhibitor Library | Alkaloid Natural Product Library | Anti-Cancer Active Compound Library | Anti-Cancer Approved Drug Library | EMA Approved Drug Library | Bioactive Compound Library
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