伊立替康盐酸盐 T0486L

Product Introduction
Bioactivity


英文名: Irinotecan Hydrochloride

描述: Irinotecan Hydrochloride (CPT-11 hydrochloride) 是喜树碱半合成衍生物的盐酸盐，是一种拓扑异构酶 I 抑制剂，可研究结肠癌和直肠癌。

细胞实验: Irinotecan hydrochloride is dissolved in DMSO and stored, and then diluted with appropriate medium before use[1]. To determine the effects of Irinotecan in combination with 5-FU, the MTT assay is used. Depending on the cell lines, 10,000 to 20,000 cells per well are seeded in 96-well plates and incubated for 24 h in complete medium. On day 2, cells are incubated in the absence or presence of Irinotecan for 30 min followed by 5-FU for 24 h. After another 24 h in complete medium without any additives, MTT reagent is added on day 4 to initiate the assay and the cells are incubated for an additional 4 h at 37°C. After removal of the medium and dissolving the crystals with acidified isopropanol, the samples are analyzed using an ELISA plate reader at 570 nm. The value at 650 nm is subtracted as background[1].

体外活性: Irinotecan Hydrochloride （0.1、1、10、100和1000 μg/mL）以剂量依赖的方式抑制所有细胞系的生长。在MTT检测中，经过90分钟的孵育后COLO-357细胞对Irinotecan Hydrochloride较为敏感，而HT29细胞不起作用。不同细胞系的Irinotecan Hydrochloride IC50浓度分别为：HT29为100 μg/mL、NMG 64/84为50 μg/mL、COLO-357为5.4 μg/mL、MIA PaCa-2为23 μg/mL以及PANC-1为46 μg/mL[1]。

体内活性: Irinotecan Hydrochloride（100 mg/kg i.p.）注射以后以剂量和时间依赖的方式影响体重变化（P<0.01）。在注射Irinotecan Hydrochloride（CPT-11）后的第 3 天和第 4 天，观察到平均最大体重减轻。注射Irinotecan Hydrochloride（CPT-11）后，17:00 时的平均体重损失最小。此外，在 5:00 或 9:00 时注射Irinotecan Hydrochloride（CPT-11）后，平均体重下降幅度最大[2]。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 62.31 mg/mL (100 mM)
H2O : 3.12 mg/mL (5 mM))


关键字: CPT 11 | Irinotecan Hydrochloride | Autophagy | CPT-11 | Inhibitor | Camptothecin 11 Hydrochloride | CPT-11 Hydrochloride | inhibit | Irinotecan | Topoisomerase | CPT11 | (+)-Irinotecan | CPT11 Hydrochloride | CPT 11 Hydrochloride

相关产品: Voreloxin | Flumequine | Heveaflavone | Topovale | Ac-Exatecan | Ellagic acid | AuM1Phe | Ciprofloxacin | NU2058 | Nelonemdaz

相关库: FDA-Approved Drug Library | Drug Repurposing Compound Library | Anti-Cancer Drug Library | Inhibitor Library | Anti-Cancer Clinical Compound Library | Anti-Colorectal Cancer Compound Library | Alkaloid Natural Product Library | Anti-Cancer Approved Drug Library | Traditional Chinese Medicine Monomer Library | Anti-Cancer Active Compound Library

伊立替康盐酸盐 T0486L信息由TargetMol中国为您提供，如您想了解更多关于伊立替康盐酸盐 T0486L报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途