4--O-甲基和厚朴酚 T10153
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mL | 68592-15-4 | ¥1,370.00 | 询底价 |
10 mg | 68592-15-4 | ¥2,160.00 | 询底价 |
2 mg | 68592-15-4 | ¥740.00 | 询底价 |
5 mg | 68592-15-4 | ¥1,230.00 | 询底价 |
Product Introduction
Bioactivity
英文名: 4-O-Methyl honokiol
描述: 4-O-Methyl honokiol, a natural neolignan isolated from Magnolia officinalis, acts as a PPARγ agonist and inhibits NF-κB activity, used for cancer and inflammation research.
细胞实验: Cells (5 × 10^4 cells per well) are plated onto 24-well plates. The cell growth inhibitory effect of 4-O-Methyl honokiol is evaluated in cells treated with 4-O-Methyl honokiol (0-30 μM) for 0-72 h, using an excluded trypan blue assay [1].
动物实验: Six-week-old male BALB/c athymic nude mice are used in the assay. SW620 and PC3 cells are injected s.c. (1 × 10^7 cells in 0.1 mL PBS per animal) into the lower right flanks of mice. After 20 days, when the tumors have reached an average volume of 300-400 mm3 or about 50 mm3, the tumor-bearing nude mice are i.p. injected with 4-O-Methyl honokiol (40 and 80 mg/kg dissolved in 0.1% DMSO) twice per week for 3 weeks. Cisplatin (10 mg/kg) is also i.p. injected once a week as a positive control. The group treated with 0.1% DMSO is designated as the control. The tumor volumes are measured with vernier calipers and calculated by the following formula: (A × B^2)/2, where A is the larger and B is the smaller of the two dimensions [1].
体外活性: 4-O-Methyl honokiol (20 μM) increases the expression, transcription, and DNA binding activities, and nuclear translocation of PPARγ in both in prostate PC-3 and LNCap cells. 4-O-Methyl honokiol (0-30 μM) inhibits LNCaP and PC-3 cancer cells growth, causes G0/G1 phase arrest, and induces apoptotic cell death, and such effects can be reversed by PPARγ antagonist. 4-O-Methyl honokiol inhibits NF-κB activity and cancer cell growth, but such effects as well as its activation of PPARγ can be abolished by knockdown of p21 [1]. 4-O-methyl honokiol (0.5, 1, and 2 μM) reduces LPS-induced release of NO, PGE2, ROS, TNF-α, and IL-1β in cultured astrocytes, and amyloidogenesis in cultured astrocytes and microglial BV-2 cells [2].
体内活性: 4-O-Methyl honokiol (40 or 80 mg/kg, i.p. every day for 4 weeks) inhibits the growth of SW620 and PC3 tumors in SW620 and PC3 xenograft model. 4-O-Methyl honokiol significantly increases the expression of p21 and PPARγ in the tumor tissues [1]. 4-O-Methyl honokiol (0.5 or 1 mg/kg/day daily for 3 weeks) significantly ameliorates LPS-induced memory impairment and inhibits LPS-induced iNOS and COX-2 expression in mice. 4-O-Methyl honokiol also shows inhibitory activities against the Aβ1-42 accumulation and activates astrocytes and microglia in LPS-injected mice brain [2].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
关键字: 4 O Methyl honokiol | 4-O-Methyl honokiol | 4OMethyl honokiol
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途