夫西地酸 T1160

Product Introduction
Bioactivity


英文名: Fusidic acid

描述: Fusidic acid (Fusidine) 是一种从 Fusidium coccineum 发酵液中分离出来的甾体类抗生素。它通过阻止核糖体释放翻译延长因子 G 来抑制细菌的生长。

激酶实验: DHODase activity is measured by the DCIP colorimetric assay. This is a coupled assay in which oxidation of DHO and subsequent reduction of ubiquinone are stoichiometrically equivalent to the reduction of DCIP. Reduction of DCIP is accompanied by a loss of absorbance at 610 nm (ε=21500 M/cm). The assay is performed in a 96-well microtiter plate at ambient temperature (ca. 25°C). Stock solutions of 10 mM leflunomide and A771726 are prepared in dimethyl sulfoxide (DMSO) and these are diluted with reaction buffer (100 mM Tris and 0.1 % Triton X-100, pH 8.0) to prepare working stocks of the inhibitors at varying concentrations. For each reaction, the well contained 10 nM DHODase, 68 μM DCIP, 0.16 mg/mL gelatin, the stated concentration of ubiquinone, 10 μL of an inhibitor working stock to give the stated final concentration, and reaction buffer. After a 5-min equilibration period, the reaction is initiated by addition of DHO to the stated final concentrations. The total volume of reaction mixture for each assay is 150 μL, and the final DMSO concentration is ≤ 0.01% (v/v). The reaction progress is followed by recording the loss of absorbance at 610 nm over a 10-min period (during which the velocity remained linear). Velocities are reported as the change in absorbance at 610 nm per minute, and each reported value is the average of three replicates. In experiments where the DHO or ubiquinone concentration is varied, the other substrate is held constant at 200 μM. To determine the inhibitor potency of leflunomide and A771726, the effects of varying concentrations of the two compounds on the initial velocity of the DHODase reaction is measured over a concentration range of 0.01?1.0 μM. In these experiments the DHO and ubiquinone concentrations are held constant at 200 and 100 μM, respectively.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 45 mg/mL (87.09 mM)


关键字: SQ16603 | elongation | inhibit | Inhibitor | Fusidate | Antibiotic | Fusidic acid | growth | Bacterial | ribosome | SQ 16603 | SQ-16603 | bacteriostatic | steroids | factor

相关产品: Fusidic acid sodium salt | Isoniazid | 2-Pyridinecarbohydrazide | Fomepizole | Ethionamide | Chlorhexidine dihydrochloride | Chlorhexidine diacetate | Chlorhexidine

相关库: Anti-Aging Compound Library | Drug Repurposing Compound Library | Approved Drug Library | Antibiotics Library | Bioactive Compounds Library Max | Human Metabolite Library | Natural Product Library for HTS | Microbial Natural Product Library | NO PAINS Compound Library | Bioactive Compound Library

夫西地酸 T1160信息由TargetMol中国为您提供，如您想了解更多关于夫西地酸 T1160报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途