叠氮胸苷 T1416

Product Introduction
Bioactivity


英文名: Zidovudine

描述: Zidovudine (ZDV) 是一种核苷逆转录酶抑制剂，有潜力研究 HIV 感染。

细胞实验: Zidovudine is prepared in DMSO (10 mM) and stored, and then diluted with appropriate medium before use[1]. Assays are performed in all cell types in the presence of titrating concentrations of ARV. 5,000 SVG, 2,500 PFA, 200,000 PBMC, or 50,000 MDM cells/well are seeded into triplicate wells of 96-well plates. Twenty-four hours later, the culture medium is removed and replaced with medium containing the ARV or DMSO (0.5% vol/vol), and equivalent TCID50 infectious units of luciferase reporter virus are added to the cells. After a 16 h incubation at 37°C, the initial viral inoculum is removed and replaced with culture medium containing the same antiretroviral drug (ARV) or DMSO (0.5% vol/vol) concentrations. At 72 h post infection, the medium is aspirated, the cells are lysed and HIV-1 infection measured using the Luciferase Assay System. Luminescence is measured using a FLUOStar Optima microplate reader. Inhibition curves and the 50% (EC50) and 90% (EC90) effective concentrations are determined by nonlinear regression analysis, using GraphPad Prism software[1].

体内活性: Intravitrous injection of the NRTIs Lamivudine (3TC), Zidovudine (AZT), or Abacavir (ABC) suppresses the laser-induced choroidal neovascularization (CNV) in wild-type mice compared to PBS vehicle. The mean level of VEGF-A in the RPE/choroid, which peaks on day 3 after laser injury, is significantly reduced in 3TC-, AZT- and ABC-treated eyes compared with control eyes in wild-type mice, but not inP2rx7-/- mice[6].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 13.4 mg/mL (50 mM)
DMSO : 26.7 mg/mL (100 mM)


关键字: HIV | CRISPR/Cas9 | inhibit | Human immunodeficiency virus | Inhibitor | NSC602670 | Zidovudine | NSC-602670

相关产品: BRD0539 | KU-57788 | L755507 | SCR7 | SCR7 pyrazine | Nocodazole | Brefeldin A

相关库: Nucleotide Compound Library | Drug Repurposing Compound Library | Anti-COVID-19 Compound Library | Cardiotoxicity Compound Library | Bioactive Compounds Library Max | CNS-Penetrant Compound Library | Inhibitor Library | EMA Approved Drug Library | Anti-Infection Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途