硫酸长春碱 T1668
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mg | 143-67-9 | ¥189.00 | 询底价 |
50 mg | 143-67-9 | ¥2,180.00 | 询底价 |
25 mg | 143-67-9 | ¥1,180.00 | 询底价 |
10 mg | 143-67-9 | ¥634.00 | 询底价 |
1 mL | 143-67-9 | ¥629.00 | 询底价 |
100 mg | 143-67-9 | ¥3,470.00 | 询底价 |
5 mg | 143-67-9 | ¥423.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Vinblastine sulfate
描述: Vinblastine sulfate (Vincaleukoblastine sulfate salt) 是对各种癌症类型有细胞毒性的生物碱。 它可以抑制微管的形成,也可以抑制 nAChR,IC50值为8.9 μM。
细胞实验: Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth. (Only for Reference)
激酶实验: Cell based receptor autophosphorylation assays: Autophosphorylation of PDGFR family kinase assays are cell-based enzyme-linked immunosorbent (ELISA) assays using CHO cells expressing wild-type PDGFRβ, chimeric protein PDGFRβ/c-Kit, and PDGFRβ/Flt3 which contain the extracellular and transmembrane domains of PDGFRβ and the cytoplasmic domain of c-Kit, and Flt-3. Cells are grown to confluency in 96-well microtiter plates under standard tissue culture conditions, followed by serum starvation for 16 hours. Briefly, quiescent cells are incubated at 37 °C with increasing concentrations of Tandutinib for 30 minutes followed by the addition of 8 nM PDGF-BB for 10 minutes. Cells are lysed in 100 mM Tris, pH 7.5, 750 mM NaCl, 0.5% Triton X-100, 10 mM sodium pyrophosphate, 50 mM NaF, 10 μg/mL aprotinin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonyl fluoride, 1 mM sodium vanadate, and the lysate is cleared by centrifugation at 15,000 g for 5 minutes. Clarified lysates are transferred into a second microtiter plate in which the wells are previously coated with 500 ng/well of 1B5B11 anti-PDGFRβ mAb and then incubated for 2 hours at room temperature. After washing three times with binding buffer (0.3% gelatin, 25 mM HEPES, pH 7.5, 100 mM NaCl, 0.01% Tween 20), 250 ng/mL of rabbit polyclonal anti-phosphotyrosine antibody is added and plates are incubated at 37 °C for 60 minutes. Subsequently, each well is washed three times with binding buffer and incubated with 1 μg/mL of horseradish peroxidase-conjugated anti-rabbit antibody at 37 °C for 60 minutes. Wells are washed prior to adding 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and the rate of substrate formation is monitored at 650 nm.
体外活性: The average terminal half-lives of Vinblastine is 14.3 h. When incubated in freshly isolated rat hepatocytes, VLB penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. Vinblastine inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. vinblastine gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].
体内活性: Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of VBL and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of vinblastine inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].
存储条件: store at low temperaturePowder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 90.9 mg/mL(100 mM)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
关键字: inhibit | Vinblastine sulfate | Vincaleukoblastine | Autophagy | Vincaleukoblastine Sulfate | Microtubule/Tubulin | Inhibitor | Vinblastine Sulfate | NSC 49842 | Vinblastine | NSC-49842
相关产品: Maytansinol | Lys-SMCC-DM1 | Tubulin inhibitor 7 | MMAF | CMPF | Deoxypodophyllotoxin | 10-Deacetyltaxol | GSK-3 inhibitor 3 | Tubulin inhibitor 35 | Entasobulin
相关库: Drug Repurposing Compound Library | Anti-Neurodegenerative Disease Compound Library | Anti-Cancer Drug Library | Microtubule-Targeted Compound Library | Membrane Protein-targeted Compound Library | Anti-Cancer Clinical Compound Library | Inhibitor Library | Traditional Chinese Medicine Monomer Library | Anti-Cancer Active Compound Library | Anti-Cancer Approved Drug Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途