盐酸强力霉素 T1687L

Product Introduction
Bioactivity


英文名: Doxycycline (hyclate)

描述: Doxycycline hyclate (WC2031) 属于四环素类抗生素，是一种广谱的金属蛋白酶 (MMP) 抑制剂，具有口服活性。Doxycycline hyclate 具有抗菌活性和抗肿瘤活性。

细胞实验: Doxycycline is diluted in culture medium at a concentration of 10 μg/mL. All in vitro treatments are performed in SMC cultures at 90% confluence, when ECM synthesis in SMC starts to be evident. Doxycycline (20 nM) is diluted in culture medium at a concentration of 10 μg/mL (20 nM), at which no toxicity or variation in primary cultured SMC proliferation has been reported, as well as in other cell lines and the incubation time is 48 h. SMC-C and SMC-Ch are seeded at equal cell density in 6-well plates and, when confluence reaches 90%, 1 mL culture medium is added to each well containing 3.7×104?Bq L-[5-3H]-proline (9.62×1011 Bq/mmol). After 48?h incubation, cells are lysed with 0.5 mL 0.5 mol/L NaOH for 1 h. The resulting solution is neutralized with an equal amount of 0.5 mol/L HCl, and 50 μL are used to measure total proteins with the Bradford method. One volume of 10% TCA is added to the remaining 250 μL and centrifuged at 13,000 g for 15?min at 4°C. The resulting precipitate is dissolved in 100 μL 0.2 mol/L NaOH, and then neutralized with 1 mol/L HCl. The solution is incubated with collagenase buffer (Tris-HCl, pH 7.6 20 mM, and CaCl2 250 mM final concentration) and 10 units of collagenase at 37°C overnight. Then, 150 μL 10% TCA are added and centrifuged at 13000 g for 15?min at 4°C. The resulting supernatant is added to 4 mL of scintillation fluid and the radioactivity is measured in a liquid scintillation counter LS 600 TA.

激酶实验: Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size.

体外活性: 方法：22 种肿瘤细胞用 Doxycycline hyclate 处理 48 h，使用 MTT 方法检测细胞活力。结果：肺癌细胞对 Doxycycline 比其他大多数细胞系更敏感。NCI-H446 和 A549 细胞对 Doxycycline 敏感，IC50分别为 1.70 μM 和 1.06 μM。[1]方法：人胰腺癌细胞 PANC-1 用 Doxycycline hyclate (10-40 μg/mL) 处理 8-24 h，使用 Flow Cytometry 方法检测细胞周期情况。结果：Doxycycline 使细胞停滞在细胞周期的 G1-S 期。[2]

体内活性: 方法：为检测体内抗肿瘤活性，将 Doxycycline hyclate (50 mg/kg) 腹腔注射给携带人乳腺癌肿瘤 4T1 的 BALB/c 小鼠，每天一次，持续十五天。结果：Doxycycline 延迟肿瘤生长并降低肿瘤相关巨噬细胞和血管的数量。[3]方法：为检测体内抗菌活性，将 Doxycycline hyclate (40 mg/kg) 腹腔注射给暴露于 B. pseudomallei 1026b 的 BALB/c 小鼠，每天两次，持续两周。结果：用 Doxycycline 治疗的小鼠 100% 存活，并且从大多数存活小鼠的肺或脾脏中没有扩增出 B. pseudomallei 的 DNA。[4]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 13.89 mg/mL
DMSO : 55 mg/mL (107.23 mM)


关键字: U343 | anti-proliferative activity | SVG | MCF 12A | WC-2031 | antibacterial activity | Matrix metalloproteinases | 293T | LNT-229 | hyclate | inhibit | Doxycycline (hyclate) | WC 2031 | Antibiotic | MMP | Doxycycline Hydrochloride | Inhibitor | Bacterial | G55 | Doxycycline

相关产品: MMP-2/MMP-9 Inhibitor I | TMI-1 | T-26c | Prinomastat | (-)-Epigallocatechin | β-Anhydroicaritin | GI254023X | Glucosamine | Fenofibric acid | MMP-3 Inhibitor

相关库: Drug Repurposing Compound Library | Polyphenolic Natural Product Library | Anti-Metabolism Disease Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Natural Product Library for HTS | EMA Approved Drug Library | Anti-Bacterial Compound Library | Microbial Natural Product Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途