化合物Tenovin-6 T1818

Product Introduction
Bioactivity


英文名: Tenovin-6

描述: Tenovin-6 是 Tenovin-1 的类似物，能抑制二氢乳清酸脱氢酶。它也抑制纯化人 SIRT1、SIRT2 和 SIRT3 蛋白脱乙酰酶活性，IC50分别为 21 μM、10 μM 和 67 μM。它是一种 p53转录活性的激活剂。

细胞实验: The MTS assay is used to evaluate cell viability. UM cells are seeded into each well of 96-well plates (5,000 cells/well) and treated the next day with control or Tenovin-6 in an increasing concentrations from 0 to 20?μM for 68?h, and then MTS is added at 20?μL/well to be read at a wave length of 490?nm, the IC50 is determined by curve fitting of the sigmoidal dose-response curve.

激酶实验: Assays are carried out using purified components in the Fluor de Lys Fluorescent Assay Systems. Relevant FdL substrates are used at 7 μM and NAD+ at 1 mM. Tenovins are solubilized in DMSO with the final DSMO concentration in the reaction being less than 0.25%. For SirT1 and HDAC8, one unit of enzyme is used per reaction, and for SirT2 and SirT3, five units is used per reaction. Reactions are carried out at 37°C for 1 hr.

体内活性: Tenovins are active on mammalian cells at one-digit micromolar concentrations and decrease tumor growth in vivo as single agents. Tenovin-6 (50 mg/kg, i.p.) inhibits the growth of tumor in mice[1].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 91 mg/mL (200.2 mM)
H2O : 90 mg/mL (198 mM)


关键字: Dihydroorotate Dehydrogenase | Inhibitor | Autophagy | MDM-2/p53 | MDM2 | Tenovin6 | acetylation | Sirtuin | degradation | DHODH | inhibit | protein-deacetylating | Tenovin-6

相关产品: 6PGD-IN-S3 | Prunetin | IDH1 Inhibitor 2 | Orotic acid | XEN723 | Nitrofurazone | LDH-IN-1 | 3-Dehydrotrametenolic acid | Qc1 | SCD1 inhibitor-3

相关库: Pyroptosis Compound Library | Transcription Factor-Targeted Compound Library | Anti-Cancer Compound Library | Mitochondria-Targeted Compound Library | Anti-Lung Cancer Compound Library | Epigenetics Compound Library | Anti-Colorectal Cancer Compound Library | PPI Inhibitor Library | DNA Damage & Repair Compound Library | NO PAINS Compound Library

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