化合物JIB04 T1868

Product Introduction
Bioactivity


英文名: JIB-04

描述: JIB-04 (NSC 693627) 是一种 Jumonji 组蛋白去甲基酶广谱抑制剂，能抑制 JARID1A、JMJD2E、JMJD3、JMJD2A、JMJD2B、JMJD2C 和 JMJD2D 的活性，其IC50值分别为 230、340、855、445、435、1100 和 290 nM。

细胞实验: For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference)

体外活性: 在H358和A549小鼠异种移植模型中,JIB-04抑制Jumonji组蛋白脱甲基酶的活性,抑制肿瘤细胞生长.

体内活性: 在肺癌和前列腺癌细胞系中（IC50=10 nM）,JIB-04下调增殖基因,阻断细胞生长。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 30.9 mg/mL (100 mM)


关键字: Apoptosis | Inhibitor | Histone Demethylase | NSC693627 | NSC-693627 | inhibit | JIB-04 | JIB04

相关产品: Ziyuglycoside II | XL019 | CDK8-IN-13 | Dasatinib monohydrate | BX-912 | AZD1208 | Garcinol | Myricetin | Rilmenidine Phosphate | SU9516

相关库: Anti-Aging Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Epigenetics Compound Library | Stem Cell Differentiation Compound Library | Reprogramming Compound Library | Methylation Compound Library | Histone Modification Compound Library | Bioactive Compound Library | Chromatin Modification Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途