化合物Inauhzin T1887

Product Introduction
Bioactivity


英文名: Inauhzin

描述: Inauhzin (INZ) 是一种新型小分子，通过抑制 SIRT1 活性有效地重新激活 p53，促进人 Y 细胞的 p53 依赖性凋亡，在 A549 细胞中的IC50为3 uM。

激酶实验: Kinase Profile Assay: Assays for serine/threonine kinases using radio labeled [γ-33P] ATP are performed in 96 well plates. BRAF and c-RAF are expressed as N-terminal FLAG-tagged protein using a baculovirus expression system. The reaction conditions are optimized for each kinase: BRAF (25 ng/well of enzyme, 1 μg/well of GST-MEK1(K96R), 0.1 μCi/well of [γ-32P] ATP, room temperature, 20 min reaction); c-RAF (25 ng/well of enzyme, 1 μg/well of GST-MEK1 (K96R), 0.1 μCi/well of [γ-32P] ATP, room temperature, 20 min reaction). Enzyme reactions are performed in 25 mM HEPES, pH 7.5, 10 mM magnesium acetate, 1 mM dithiothreitol and 0.5 μM ATP containing optimized concentration of enzyme, substrate and radiolabeled ATP as described above in a total volume of 50 μL. Prior to the kinase reaction, compound and enzyme are incubated for 5 min at reaction temperature as described above. The kinase reactions are initiated by adding ATP. After the reaction period as described above, the reactions are terminated by the addition of 10% (final concentration) trichloroacetic acid. The [γ-33P] or [γ-32P]-phosphorylated proteins are filtered in GFC filter plates with a Cell Harvester and then the plates are washed out with 3% phosphoric acid. The plates are dried, followed by the addition of 40 μL of MicroScint0. The radioactivity is counted by a TopCount scintillation counter.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 21 mg/mL


关键字: Sirtuin | Inhibitor | Inauhzin | MDM-2/p53 | inhibit

相关产品: OSS_128167 | AK-7 | Tenovin-6 | MDL-811 | SIRT1-IN-1 | JGB1741 | Suramin Sodium Salt | Salvianolic acid B | YK-3-237 | RK-9123016

相关库: Anti-Aging Compound Library | HIF-1 Signaling Pathway Compound Library | Anti-Lung Cancer Compound Library | Ferroptosis Compound Library | Inhibitor Library | Epigenetics Compound Library | PPI Inhibitor Library | Target-Focused Phenotypic Screening Library | Stem Cell Differentiation Compound Library | Histone Modification Compound Library

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