钙黄绿素-AM T18931

Product Introduction
Bioactivity


英文名: Calcein-AM

描述: Calcein-AM is a cell-permeable fluorescent dye used for the determination of cell viability.

体外活性: The calcein-AM dye used to stain the living cells have a low spontaneous leakage rate of less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM have a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this micro-test. Quantitation of killing and kinetic analysis is readily performed with the test system [1]. Calcein-AM is pH-independent, better retained, and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader [2]. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with the intact plasma membrane. The calcein-AM assay has been used to assess cell viability, cytotoxicity, and tp quantitate apoptosis [3].

存储条件: keep away from direct sunlightPowder: -20°C for 3 years | In solvent: -80°C for 1 year

关键字: CalceinAM | Calcein AM | Calcein-AM

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途