化合物JSH-23 T1930

Product Introduction
Bioactivity


英文名: JSH-23

描述: JSH-23 是NF-κB 抑制剂，作用于脂多糖刺激的巨噬细胞 RAW 264.7，抑制 NF-κB 转录活性(IC50=7.1 μM)，抑制 NF-κB p65 的核易位而不影响 IκBα 降解。

细胞实验: Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference)

激酶实验: Measurement of NF-κB transcriptional activity: Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.

体外活性: 在糖尿病大鼠体内,JSH-23（3 mg/kg）能够减少神经炎症,并提高抗氧化防御明显逆转神经传导,最终改善神经血流量不足.

体内活性: 在RAW 264.7细胞中,JSH-23<100 μM时没有表现出明显的毒性作用,同时能够抑制脂多糖诱导的凋亡染色质浓缩。在老鼠小脑原代细胞中,JSH-23抑制脂多糖诱导的NF-κB p65核转运,而不影响IκBα降解。

存储条件: store at low temperaturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (208.04 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 16 mg/mL (66.6 mM)


关键字: JSH-23 | Nuclear factor-κB | Nuclear factor-kappaB | NF-κB | Inhibitor | JSH 23 | JSH23 | inhibit

相关产品: Chicanine | Sodium lauryl sulfoacetate | Pimaric acid | Stachydrine | Nepetoidin B | Glabrol | Gliotoxin | Ethyl gallate | 7,8-Didehydrocimigenol | CORM-3

相关库: Antioxidant Compound Library | Anti-Aging Compound Library | HIF-1 Signaling Pathway Compound Library | Transcription Factor-Targeted Compound Library | Pyroptosis Compound Library | Anti-Lung Cancer Compound Library | Bioactive Compounds Library Max | Ferroptosis Compound Library | Inhibitor Library | Anti-Pancreatic Cancer Compound Library

化合物JSH-23 T1930信息由TargetMol中国为您提供，如您想了解更多关于化合物JSH-23 T1930报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途