2-硝基苯-beta-D-半乳糖苷 T19497

Product Introduction
Bioactivity


英文名: ONPG

描述: ONPG (2-Nitrophenyl β-D-galactopyranoside) 是用作比色和分光光度计底物，可用作 β-半乳糖苷酶活性的检测。

体外活性: The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (Km), turnover numbers (kcat), and catalytic efficiencies (kcat/Km) of Gal308 for ONPG and lactose are determined. The kcat and Km values are 464.7±7.8 s-1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s-1 and 7.1±0.8 mM for lactose, respectively. The kcat/Km value of the enzyme for ONPG (172.1 s-1mM-1) is 4.6-fold higher than that for lactose (37.2 s-1mM-1), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose[1].

存储条件: store at low temperaturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 7.4 mg/mL (24.56 mM)


关键字: inhibit | 2-Nitrophenyl b-D-galactopyranoside | Inhibitor | ONPG | 2-Nitrophenyl beta-D-galactopyranoside

相关库: Bioactive Compounds Library Max | Bioactive Compound Library

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