化合物PSI6206 T2117
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
50 mg | 863329-66-2 | ¥793.00 | 询底价 |
100 mg | 863329-66-2 | ¥1,180.00 | 询底价 |
5 mg | 863329-66-2 | ¥228.00 | 询底价 |
1 mg | 863329-66-2 | ¥113.00 | 询底价 |
10 mg | 863329-66-2 | ¥328.00 | 询底价 |
1 mL | 863329-66-2 | ¥293.00 | 询底价 |
25 mg | 863329-66-2 | ¥547.00 | 询底价 |
Product Introduction
Bioactivity
英文名: PSI-6206
描述: PSI-6206 (RO 2433) 是 PSI-6130 的脱氨衍生物,PSI-6206 是一种选择性 HCV RNA 聚合酶抑制剂,低效抑制HCV 复制,EC90为 >100 μM。PSI-6130 是一种有效的选择性 HCV NS5B 聚合酶抑制剂。
激酶实验: Protein Kinase Assays: The in vitro kinase assays are performed in 96-well plates (30 μL) at ambient temperature for 15–45 min using the recombinant glutathione S-transferase-fused kinase domains (4-100 ng, depending on specific activity). [γ33P]ATP is used as phosphate donor and polyGluTyr-(4:1) peptide as acceptor. With the exception of protein kinase C-α, cyclin-dependent kinase 1/cycB and protein kinase A are protamine sulfate (200 μg/mL), histone H1 (100 μg/mL), and the heptapeptide Leu-Arg-Arg-Ala-Ser-Leu-Gly (known as Kemptide Bachem) respectively and are used as peptide substrates. Assays are optimized for each kinase using the following ATP concentrations: 1.0 μM (c-Kit, c-Met, c-Fms, c-Raf-1, and RET), 2.0 μM (EGFR, erbB2, ErbB3, and ErbB4), 5.0 μM (c-abl), 8.0 μM (Flt-1, Flt-3, Flt-4, Flk, KDR, FGFR-1, and Tek), 10.0 μM (PDGFR-β, protein kinase C-α, and cyclin-dependent kinase 1), and 20.0 μM (c-Src and protein kinase A). The reaction is terminated by the addition of 20 μL 125 mM EDTA. Thirty μL (c-abl, c-Src, insulin-like growth factor-1R, RET-Men2A, and RET-Men2B) or 40 μL (all other kinases) of the reaction mixture is transferred onto Immobilon-polyvinylidene difluoride membrane, presoaked with 0.5% H3PO4 and mounted on a vacuum manifold. Vacuum is then applied and each well rinsed with 200 μL 0.5% H3PO4. Membranes are removed and washed four times with 1.0% H3PO4 and once with ethanol. Dried membranes are counted after mounting in a Packard TopCount 96-well frame and with the addition of 10 μL/well of Microscint. IC50 values (±SE) are calculated by linear regression analysis of the percentage inhibition and are averages of at least three determinations.
体外活性: PSI-6206 (RO2433) is tested for anti-HCV activity in both a cell-based quantitative real-time RT-PCR assay and surrogate bovine viral diarrhea virus (BVDV) assays and demonstrates no activity or cytoxicity in any assay[1]. The formation of the 5′-triphosphate (TP) of PSI-6130 (PSI-6130-TP) and RO2433 (RO2433-TP) increases with time and reached steady state levels at 48 h. RO2433-TP also inhibits RNA synthesis by the native HCV replicase isolated from HCV replicon cells and the recombinant HCV polymerase NS5B[2]. PSI-6206 (RO2433) is the deaminated derivative of PSI-6130, which is a potent and selective inhibitor of HCV NS5B polymerase[3].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 60 mg/mL (230.57 mM)
关键字: Inhibitor | Hepatitis C virus | PSI 6206 | RO-2433 | inhibit | RO2433 | PSI6206 | PSI-6206 | GS 331007 | HCV | GS331007
相关产品: AG-1478 | Clemizole hydrochloride | GS-443902 | Ledipasvir | Glecaprevir | Setrobuvir | Methyl 2-amino-5-bromobenzoate | Sofosbuvir impurity N | 2',5-Difluoro-2'-deoxycytidine | Peretinoin
相关库: Nucleotide Compound Library | Anti-Aging Compound Library | Anti-COVID-19 Compound Library | Mini Electrophilic Heterocyclic Fragment Library | Bioactive Compounds Library Max | Inhibitor Library | Featured Fragment Library | High Solubility Fragment Library | Anti-Viral Compound Library | Anti-Infection Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途