山奈酚 T2177

Product Introduction
Bioactivity


英文名: Kaempferol

描述: Kaempferol (Robigenin) 属于天然类黄酮，是一种 ERRα 和 ERRγ 的反向激动剂。Kaempferol 具有抗肿瘤、抗炎、抗氧化、抗菌和抗病毒等多种活性。

细胞实验: Kaempferol is prepared in DMSO (100 mM) and stored (-20°C), and then diluted with appropriate medium[2]. Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160 μM Kaempferol for 24 hours in triplicates. The medium is removed, and the plates are freeze-thawed to lyse cells. Each well is added with 200 μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50 μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a real-time Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers, a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate, and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis[2].

激酶实验: Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50 mM Tris-HCl pH 7.5, 100 mM KCl, 1 mM ethylenediamine tetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, 0.5 mM Benzamidine, 20 mg/L Leupeptin, 20 mM sodium pyrophosphate, 50 mM NaF, and 50 mM sodium β-glycerophosphate), and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit[3].

体外活性: 方法：人乳腺癌细胞 MDA-MB-231 用 Kaempferol (0.01-100 μM) 处理 72 h，使用 MST assay 检测细胞活力。结果：Kaempferol 以剂量依赖的方式抑制细胞增殖，IC50 值为 43 μmol/L。[1]方法：人胃癌细胞 AGS 和 SNU638 用 Kaempferol (50 μM) 处理 8-24 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果：Kaempferol 以时间依赖的方式增加 cleaved caspase-3 和 -9 并降低 Bcl-2 水平。[2]

体内活性: 方法：为研究对失血性休克小鼠的影响，将 Kaempferol (10 mg/kg) 腹腔注射给经历失血性休克的 C57/BL6 的小鼠。结果：Kaempferol 预处理失血性休克小鼠可显著降低血浆 TNF-α 和 IL-6 水平；恢复了心、肺和肝的 MPO、SOD 和 MDA；并且增加了 HO-1 在相同器官中的表达。[3]方法：为研究抗神经炎症作用，将 Kaempferol (25-100 mg/kg) 灌胃给药给 BALB/c 小鼠，每天一次，持续七天，第七天注射 LPS (5 mg/kg)。结果：Kaempferol 可能是一种很有前途的神经保护剂，通过抑制 HMGB1 的释放和下调 TLR4/MyD88 炎症途径来缓解炎症反应和血脑屏障功能障碍。[4]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 28.6 mg/mL (100 mM)
DMSO : 45 mg/mL (157.21 mM)


关键字: Kaempferol | Parasite | inhibit | Autophagy | Endogenous Metabolite | Apoptosis | Estrogen Receptor/ERR | Mitophagy | Human immunodeficiency virus | HIV | Mitochondrial Autophagy | Inhibitor

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相关库: Tobacco Monomer Library | Anti-Metabolism Disease Compound Library | Human Metabolite Library | Anti-Ovarian Cancer Compound Library | Traditional Chinese Medicine Monomer Library | Anti-Parasitic Compound Library | Anti-Cancer Active Compound Library | Miao medicine Compound Library | Antiparasitic Natural Product Library | RO5 Drug-like Natural Product Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途