雷公藤甲素 T2179

Product Introduction
Bioactivity


英文名: Triptolide

描述: Triptolide (PG490) 属于三环二萜类天然产物，是一种 NF-κB 活化的抑制剂。Triptolide 具有免疫抑制、抗风湿、抗炎、抗增殖和抗肿瘤活性。

细胞实验: Triptolide is dissolved in DMSO (1 mg/mL) and stored, and then diluted with RPMI 1640 medium before use[3]. The viability of differentiated PC12 cells treated with different concentrations of Triptolide. After differentiated PC12 cells are cultured on 96-well plates with RPMI 1640 medium for stabilization, differentiated PC12 cells are incubated with different concentrations of Triptolide (0.01, 0.1, and 1 nM) for 24 hours. The concentrations in this study are chosen. Then cell viability is determined by the MTT assay. Each condition and experiment is repeated three times[3].

体外活性: 方法：神经母细胞瘤细胞 BE(2)-C 用 Triptolide (5-100 nM) 处理 24 h，使用 CCK-8 Assay 检测细胞活力。结果：在 BE(2)-C 细胞中观察到对 Triptolide 的浓度依赖性反应。在 50 nM 的 Triptolide 下，细胞活力显著降低至 50%。[1]方法：乳腺癌细胞 MCF-7 用 Triptolide (10-50 nmol/L) 处理 24 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果：Triptolide 以剂量依赖的方式降低 MCF-7 细胞中 ERα 的信使核糖核酸水平和蛋白质水平。[2]

体内活性: 方法：为检测体内抗肿瘤活性，将 Triptolide (0.4 mg/kg) 灌胃给药给携带乳腺癌肿瘤 MCF-7 的 BALB/c-nu+/nu+ 小鼠，每天一次，持续三周。结果：Triptolide 在小鼠模型中抑制 MCF-7 细胞异种移植物的生长。[2]方法：为研究对溃疡性结肠炎 (UC) 的作用，将 Triptolide (0.1-0.4 mg/kg) 口服给药给 DSS 诱导 UC 的小鼠，每天一次，持续七天。结果：Triptolide 对 UC 小鼠具有抗炎和治疗作用。[3]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (138.73 mM)
5% DMSO+95% Saline : 0.36 mg/mL (1 mM, suspension)


关键字: Nuclear factor-κB | NSC163062 | Triptolide | NSC-163062 | PG 490 | Apoptosis | Inhibitor | Nuclear factor-kappaB | inhibit | PG-490 | NF-κB

相关产品: Dapivirine | KRIBB11 | 3BDO | Dubermatinib | HFY-4A | TIC10 | Supinoxin | Voreloxin hydrochloride | Verbascoside | Glutathione arsenoxide hydrochloride

相关库: Drug Repurposing Compound Library | Anti-Colorectal Cancer Traditional Chinese Medicine Compound Library | Anti-Cancer Drug Library | Inhibitor Library | Anti-Cancer Clinical Compound Library | Cytoskeletal Signaling Pathway Compound Library | Anti-Cancer Active Compound Library | Traditional Chinese Medicine Monomer Library | Anti-Cancer Approved Drug Library | Covalent Inhibitor Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途