化合物SantacruzaMate A T2266

Product Introduction
Bioactivity


英文名: SantacruzaMate A

描述: SantacruzaMate A (CAY-10683) 是一种高效选择性的HDAC2抑制剂，IC50为 119 pM。

细胞实验: HuT-78 cells incubated in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS, 1% penicillin/streptomycin, and 1% L-glutamine. HCT-116 cells cultivated using McCoy’s 5A medium supplemented with 10% FBS, 1% penicillin/streptomycin, and 1% nonessential amino acids. Cells were seeded in a 96-well plate at 5000cells per well. Before treatment, the plates were incubated at 37°C, 5% CO2 for 24 h. Treatment with inhibitors were incubated in wells for 72 or 96 h using SAHA as a positive control. Antiproliferative activity was determined using a standard MTS-PMS assay. (Only for Reference)

激酶实验: HDAC Enzyme Assay: The commercially availablae human recombinant enzyme and fluorogenic HDAC assay kits have used to measure percent inhibition and IC50 values of three HDAC isozymes (HDAC2, HDAC4, HDAC6). Briefly, the inhibitor is added sequentially to a black, flat-bottom 96-well microtiter plate, and the reaction mixture is incubated for 30 min at 37°C. The potent HDAC inhibitor trichostatin A (included in the assay kit) is added to the bifunctional HDAC assay developer at a final reaction concentration of 1 μM to stop deacetylation and initiate the release of the fluorophore. The reaction mixture is further incubated at room temperature for 15 min. Fluorescence is measured on a Spectra Max Gemini XPS using an excitation wavelength of 360 nm and a detection wavelength of 460 nm.

体内活性: Santacruzamate A对HCT116结肠癌细胞和HuT-78皮肤T淋巴瘤细胞的生长有抑制作用,GI50值分别为29.4 μM 和 1.4 μM。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 51 mg/mL (183.2 mM)
Ethanol : 51 mg/mL (183.2 mM)


关键字: Inhibitor | SantacruzaMate A | inhibit | Histone deacetylases | CAY10683 | HDAC | CAY 10683

相关产品: MC1568 | Marein | Citarinostat | HDAC6-IN-22 | Droxinostat | HNHA | Scriptaid | UF010 | Domatinostat | LMK-235

相关库: Anti-Aging Compound Library | Inhibitor Library | Highly Selective Inhibitor Library | DNA Damage & Repair Compound Library | Natural Product Library for HTS | Anti-Pancreatic Cancer Compound Library | Microbial Natural Product Library | Target-Focused Phenotypic Screening Library | Histone Modification Compound Library | Natural Product Library

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