化合物CFTRinh-172 T2355

Product Introduction
Bioactivity


英文名: CFTR(inh)-172

描述: CFTR(inh)-172 (CFTR Inhibitor-172) 是一种不依赖电压的选择性 CFTR 抑制剂，在2分钟内可逆地抑制CFTR 短路电流的Ki 值为300 nM。

细胞实验: Cell toxicity is assayed by the dihydrorhodamine method at 24 hours after cell incubation with 0–1,000 μM inhibitor. (Only for Reference)

激酶实验: Screening procedures: Assays are done using a customized screening system consisting of a 3-meter robotic arm, CO2 incubator, plate washer, liquid-handling workstation, bar code reader, delidding station, and two FLUOstar fluorescence platereaders, each equipped with two syringe pumps and HQ500/20X (500 ± 10 nm) excitation and HQ535/30M (535 ± 15 nm) emission filters. The robotic system is integrated using SAMI version 3.3 software modified for two platereaders. Custom software is written in Microsoft VBA (Visual Basic for Applications) to compute base-line–subtracted, normalized fluorescence slopes (giving halide influx rates) from stored data files. The assay is set up by loading the incubator (37°C, 90% humidity, 5% CO2) with 40–60 96-well plates containing the FRT cells, and loading a carousel with 96-well plates containing test compounds and disposable plastic pipette tips. To initiate the assay, each well of a 96-well plate is washed three times in PBS (300 μl/wash), leaving 50 μL PBS. Ten microliters of a CFTR-activating cocktail (5 μM forskolin, 100 μM IBMX, 25 μM apigenin in PBS) is added, and after 5 minutes one test compound (0.5 μL of 1 mM DMSO solution) is added to each well to give 10 μM final concentration. After 10 minutes, 96-well plates are transferred to a platereader for fluorescence assay. Each well is assayed individually for CFTR-mediated I– transport by recording fluorescence continuously (200 ms per point) for 2 seconds (base line) and then for 12 seconds after rapid (<0.5 seconds) addition of 165 μL of isosmolar PBS in which 137 mM Cl– was replaced by I–.

体外活性: 6小时20 μg CFTRinh-172完全消除霍乱弧菌诱导的肠液分泌而不影响体内霍乱弧菌的生长.

体内活性: CFTRinh-172作为一种选择性的CFTR通道抑制剂,能够完全消除兔泪腺兔腺泡和导管细胞中的Cl-电流。CFTRinh-172时间和剂量依赖性的抑制了CFTR介导的碘离子转运,并且有效抑制多种激动剂或激活剂的CFTR激活。CFTRinh-172不依赖CFTR抑制而诱导ROS产生,线粒体衰竭和NF-κB信号传导途径的激活。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 40.9 mg/mL (100 mM)


关键字: CFTR(inh)-172 | Cystic fibrosis transmembrane conductance regulator | Inhibitor | CFTR(inh) 172 | CFTR(inh)172 | CFTR | inhibit | Autophagy

相关产品: Icenticaftor | Ivacaftor | GLPG1837 | UCCF-853 | DNDS | VRT-532 | Glibenclamide | CFTR corrector 4 | (R)-Elexacaftor | Cavosonstat

相关库: Ion Channel Inhibitor Library | Anti-Cancer Compound Library | Membrane Protein-targeted Compound Library | Bioactive Compounds Library Max | Fluorochemical Library | Inhibitor Library | Autophagy Compound Library | Target-Focused Phenotypic Screening Library | Covalent Inhibitor Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途