化合物BIBR 1532 T2380
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
10 mg | 321674-73-1 | ¥693.00 | 询底价 |
1 mg | 321674-73-1 | ¥193.00 | 询底价 |
1 mL | 321674-73-1 | ¥498.00 | 询底价 |
5 mg | 321674-73-1 | ¥433.00 | 询底价 |
500 mg | 321674-73-1 | ¥8,860.00 | 询底价 |
100 mg | 321674-73-1 | ¥3,970.00 | 询底价 |
50 mg | 321674-73-1 | ¥2,680.00 | 询底价 |
25 mg | 321674-73-1 | ¥1,380.00 | 询底价 |
Product Introduction
Bioactivity
英文名: BIBR 1532
描述: BIBR 1532 是一种选择性端粒酶非竞争性抑制剂,IC50值为 100 nM。
细胞实验: Cells are plated as triplicates in complete RPMI 1640 medium with various concentrations of BIBR1532. After 24 to 72 hours, water-soluble tetrazolium (WST-1) is added, which is transformed into formazan by mitochondrial reductase systems. The increase in the number of viable cells results in an increase of activity of mitochondrial dehydrogenases, leading to an increase of formazan dye formed, which is quantified by ELISA reader after 2, 3, and 4 hours of incubation. (Only for Reference)
激酶实验: Conventional Telomerase Assay : For the direct telomerase assay with the endogenous telomerase, 10 μL of telomerase-enriched extract is mixed with different concentrations of BIBR1532 in a final volume of 20 μL. After 15-minute preincubation on ice, 20 μL of the reaction mixture is added, and the reaction is initiated by transferring the tubes to 37 °C. The final concentrations in the reaction mixture are 25 mM Tris-Cl (pH 8.3), 1 mM MgCl2, 1 mM EGTA, 1 mM dATP, 1 mM dTTP, 6.3 μM cold dGTP, 15 μCi [α-32P]dGTP (3000 Ci/mmol; NEN), 1.25 mM spermidine, 10 units of RNasin, 5 mM 2-mercaptoethanol, and 2.5 μM TS-primer (5
体内活性: 在MCF-7/WT和抗美法仑的MCF-7/MlnR 细胞系中,BIBR 1532(2.5 μM)通过抑制端粒酶活性降低形成集落的能力,并缩短端粒长度,且诱导对化疗增敏。在体外,BIBR 1532剂量依赖性地非竞争性抑制端粒酶活性(IC50:100 nM)。BIBR 1532对T细胞幼淋巴细胞白血病具有选择性细胞毒性,该毒性具有剂量依赖性,BIBR 1532处理过的细胞也出现核浓缩及凋亡小体形成现象。在JVM13白血病细胞系中,BIBR 1532具有剂量依赖性的抗增殖效果(IC50:52 μM),且对其他白血病细胞系,包括 Nalm-1,HL-60和Jurkat的结果相似。此外, 在急性髓细胞性白血病中,BIBR 1532具有抗增殖效果(IC50:56 μM),而且不影响正常造血干细胞的增殖能力。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 33.1 mg/mL (100 mM)
Ethanol : 8.3 mg/mL (25 mM)
关键字: inhibit | BIBR 1532 | Inhibitor | Apoptosis | Telomerase | BIBR-1532 | BIBR1532
相关产品: Dienogest | DC661 | EP12 | Flurochloridone | Prexasertib dihydrochloride | PI-273 | PCC0208017 | Coenzyme Q9 | Sanguinarine chloride | Rasagiline Mesylate
相关库: Anti-Aging Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Highly Selective Inhibitor Library | DNA Damage & Repair Compound Library | Apoptosis Compound Library | Target-Focused Phenotypic Screening Library | Covalent Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library
化合物BIBR 1532 T2380信息由TargetMol中国为您提供,如您想了解更多关于化合物BIBR 1532 T2380报价、型号、参数等信息,欢迎来电或留言咨询。
注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途