化合物INH1 T2502

Product Introduction
Bioactivity


英文名: INH1

描述: INH1 (IBT13131) 通过直接结合Hec1，特异性干扰 Hec1/Nek2 的相互作用。它在体内外均表现出良好的抗肿瘤活性。

细胞实验: Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference)

激酶实验: Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments).

体外活性: 腹腔注射100 mg/kgINH1抑制含有MDA-MB-468人乳腺癌异种移植物的小鼠中乳腺肿瘤生长.

体内活性: INH1有效抑制人乳腺癌细胞增殖,GI50为10-21 μM。此外,INH1也会通过损害纺锤体监测点调控的Hec1/Nek2通路,引发细胞杀伤活性。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 30.8 mg/mL (100 mM)
Ethanol : 3.1 mg/mL (10 mM)


关键字: Apoptosis | inhibit | INH 1 | INH1 | Inhibitor | IBT-13131 | INH-1 | IBT 13131

相关产品: SB 202190 | PF-3758309 hydrochloride | ONO-4059 analog | Raddeanin A | Tricetin | Epothilone A | BMS-202 | DIM-C-pPhOH | PAC-1 | Pim-1 kinase inhibitor 4

相关库: Anti-Cancer Compound Library | Microtubule-Targeted Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Cytoskeletal Signaling Pathway Compound Library | Anti-Cancer Active Compound Library | PPI Inhibitor Library | Apoptosis Compound Library | NO PAINS Compound Library | Bioactive Compound Library

化合物INH1 T2502信息由TargetMol中国为您提供，如您想了解更多关于化合物INH1 T2502报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途