去氧胆酸 T2965
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
500 mg | 83-44-3 | ¥594.00 | 询底价 |
1 mL | 83-44-3 | ¥445.00 | 询底价 |
100 mg | 83-44-3 | ¥415.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Deoxycholic acid
描述: Deoxycholic acid (Cholanoic Acid) 是 G 蛋白偶联胆汁酸受体 TGR5的激活剂。
细胞实验: Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining. (Only for Reference)
体外活性: Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1].
体内活性: miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 55 mg/mL (140.1 mM)
Ethanol : 56 mg/mL (142.6 mM)
H2O : < 1 mg/mL (insoluble or slightly soluble)
关键字: TGR5 | G-protein coupled receptor 19 | G protein-coupled Bile Acid Receptor 1 | GPBAR1 | G protein-coupled bile acid receptor | GPCR19 | bile acid | inhibit | Inhibitor | Endogenous Metabolite | Deoxycholic acid
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途