查耳酮 T2S0961

Product Introduction
Bioactivity


英文名: Chalcone

描述: Chalcone (Cinnamophenone) 是从甘草中分离得到的一种芳香酮，可合成查耳酮衍生物。它具有多种生物和药理的活性，如抗炎、抗菌、抗癌、抗氧化、抗寄生虫等活性。

细胞实验: Human bladder carcinoma cell line 5637 was maintained in RPMI1640 medium supplemented with 10% fetal calf serum (FCS), 1 mM L-glutamine, and antibiotics. Cells were grown at 37°C in a humidified 5% CO2 atmosphere. For all experiments, confluent cells were trypsinized, and aliquots of single-cell suspension were re-seeded at 0.5×10^6 cells/dish (100-mm diameter) or 0.1×106 cells/dish (60-mm diameter) and cultured in poly-L-lysine-coated new culture dishes containing normal culture media for 24-48 hours. After the normal growth treatment, cells were treated under several different conditions as specified in the text. When cell number and cell viability were determined, both attached cells and unattached cells were collected by trypsinization and sedimentation of the culture media, respectively, and combined in one tube. The cells were washed twice with phosphate-buffered saline (PBS) and aliquots of the cells were stained with Trypan Blue solution. After the staining, cells were mounted in a hemocytometer for microscopic observation. Total cell number was determined by counting more than 300 cells per condition. The percentage of Trypan-Blue-stained cells (versus total cell number) was determined by calculation of data obtained with three independent counting of more than 100 cells. When nuclear morphology was evaluated, cells were grown and treated in glass-bottom dishes. After treatment, the cells were washed three times with PBS, fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.2% Triton X-100 in PBS for 2 minutes and blocked with 3% BSA in PBS for 1 hour. Cells were then treated with propidium iodide at 2 μg/ml in PBS for 1 hour. After washing with PBS (three times 5 minutes), samples were mounted in PBS containing 50 mg/ml 1,4-diazabicyclo[2,2,2]octane and 90% glycerol and subjected to confocal microscopic observation. The fluorescence was detected at 543 nm argon excitation using optics of a 590 nm long-pass barrier filter.The fluorescent images were recorded and reconstituted with the use of software Adobe Photoshop CS [2].

体外活性: AG99, a potent inhibitor of EGFR, was shown to inhibit the appearance of pp145 to some extent. AG99 promotes cell death of normal and cancer cells via activation of caspase-like proteases in vitro [2].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 55 mg/mL (264.09 mM)
H2O : 4.8 mM


关键字: anti-parasitic | anti-inflammatory | b-phenylacrylophenone | beta-phenylacrylophenone | Chalcone | antibacterial | Inhibitor | Bacterial | antioxidative | anticancer | inhibit

相关产品: EGFR/ErbB-2 inhibitor-1 | EGFR-IN-1 | Benidipine hydrochloride | Cetuximab | Pyrotinib dimaleate | EGFR-IN-82 | Tucatinib | Almonertinib | BGB-102 | EGFR-IN-88

相关库: Tobacco Monomer Library | Membrane Protein-targeted Compound Library | Kinase Inhibitor Library | Traditional Chinese Medicine Monomer Library | Anti-Parasitic Compound Library | Tyrosine Kinase Inhibitor Library | Anti-Cancer Active Compound Library | Antiparasitic Natural Product Library | Flavonoid Natural Product Library | NO PAINS Compound Library

查耳酮 T2S0961信息由TargetMol中国为您提供，如您想了解更多关于查耳酮 T2S0961报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途