松三糖 T3016

Product Introduction
Bioactivity


英文名: D-(+)-Melezitose hydrate

描述: D-(+)-Melezitose hydrate (melicitose) 是由许多以植物汁液为食的昆虫通过酶反应产生的一种非还原性三糖，能鉴定吲哚阳性和吲哚阴性克雷伯菌属的临床分离株。

激酶实验: FlashPlate assay (96-well screening assay): To columns 1 through 10, 1 μL of Olaparib (in DMSO) is added, and 1 μL DMSO only is added to the positive (POS) and negative (NEG) control wells (columns 11 and 12, respectively) of a pretreated FlashPlate. PARP-1 is diluted 1:40 in buffer (buffer B: 10% glycerol (v/v), 25 mM HEPES, 12.5 mM MgCl2,50 mM KCl, 1 mM DTT, 0.01% NP-40 (v/v), pH 7.6) and 40 μL added to all 96 wells (final PARP-1 concentration in the assay is ~1 ng/μL). The plate is sealed and shaken at RT for 15 min. Following this, 10 μL of positive reaction mix (0.2 ng/μL of double-stranded oligonucleotide [M3/M4] DNA per well, 5 μM of NAD+ final assay concentration, and 0.075 μCi 3H-NAD+ per well) is added to the appropriate wells (columns 1-11). The negative reaction mix, lacking the DNA oligonucleotide, is added to column 12 (with the mean negative control value used as the background). The plate is resealed and shaken for a further 60 min at RT to allow the reaction to continue. Then, 50 μL of ice-cold acetic acid (30%) is added to each well to stop the reaction, and the plate is sealed and shaken for a further 60 min at RT. Tritiated signal bound to the FlashPlate is then determined in counts per minute (CPM) using the TopCount plate reader.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 60 mg/mL (114.84 mM)


关键字: Inhibitor | Bacterial | (+)-Melezitose | D(+)Melezitose hydrate | D (+) Melezitose hydrate | inhibit | D-Melezitose Hydrate

相关产品: Sulfogaiacol | Chebulinic acid | Tanespimycin | Antibacterial compound 1 | Nonacosane | Rhapontigenin | Aloin(mixture of A&B) | Abietic Acid | Oxolinic acid | Ginkgolic Acid (C13:0)

相关库: Selected Plant-Sourced Compound Library | Saccharide and Glycoside Natural Product Library | Bioactive Compounds Library Max | Human Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Natural Product Library for HTS | Anti-Bacterial Compound Library | Anti-Infection Compound Library | Bioactive Compound Library | Anti-infective Natural Product Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途