油菜素内酯 T3151

Product Introduction
Bioactivity


英文名: Brassinolide

描述: Brassinolide (Brassin lactone) 是一种植物生长调节剂，可调节植物细胞伸长。

细胞实验: Brassinolide is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide[2].

体外活性: Brassinolide is a plant sterol isolated from the pollen of rape (Brassica napus L.) initially. Brassinolide can induce a time and concentration-dependent cytotoxicity in PC-3 cells. The mode of cell death appears to be predominately apoptosis, as shown by flow-cytometric analysis, fluorescence and transmission electron microscopes. Caspase-3 activity is obviously increased after Brassinolide treatment. Western blot studies indicate that treatment with Brassinolide triggered a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2, which suggests that Brassinolide can induce cytotoxicity in PC-3 cells by triggering apoptosis. Brassinolide might therefore be a promising candidate for the treatment of prostate cancer[1]. Brassinolide is a plant growth modulator, on multidrug resistance (MDR) of human T lymphoblastoid cell line CCRF-VCR 1000 which is obtained by progressively addition of vincristine (VCR) to sensitive CCRF-CEM cells, and to explore preliminarily the mechanism of reversing action. After treatment of Brassinolide under the concentration of 0.001-10 μg/mL, the resistance of CCRF-VCR is reversed partly with the reversing folds respectively as 4.4-11.6. The intracellular accumulation of rhodamine 123 is significantly reduced in the resistant cells. After treatment of Brassinolide, the accumulation increased, the level of fluorescent dye is situated between resistant cells and sensitive cells. No alteration of the catalytic activity of topoisomerase II is found among three groups. The level of protein expression of p53 in resistant cells is higher than that of sensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level of sensitive cells. Brassinolide can effectively reverse the resistance of CCRF-VCR cells by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing MDR for Brassinolide[2].

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 60 mg/mL (124.82 mM)


关键字: Inhibitor | Brassinolide | inhibit | Apoptosis

相关产品: MN58b | CHS-828 | Sildenafil citrate | Pemetrexed disodium hemipenta hydrate | SR-4835 | GSK2795039 | MKC-1 | DPN | Epirubicin hydrochloride | Acyclovir

相关库: Anti-Tumor Natural Product Library | Tobacco Monomer Library | Selected Plant-Sourced Compound Library | Bioactive Compounds Library Max | Human Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Traditional Chinese Medicine Monomer Library | Apoptosis Compound Library | Natural Product Library for HTS | Natural Product Library

油菜素内酯 T3151信息由TargetMol中国为您提供，如您想了解更多关于油菜素内酯 T3151报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途