二氢欧山芹醇当归酸酯 T3373

Product Introduction
Bioactivity


英文名: Columbianadin

描述: Columbianadin (Zosimin) 是来自当归的根的主要生物活性成分之一，是一种天然香豆素，具有抗炎和抗癌作用。

激酶实验: Cells are treated with various concentrations of Dihydrotanshinone I (3.13-20 μM) for 48 h. For the activity assay, Ac-DEVD-AMC (1 μg/μL), Ac-IETD-AMC (1 μg/μL) or Ac-LEDH-AMC (1 μg/μL) and cell lysate are added into Protease Assay Buffer in 96-well plate. Reaction mixtures with lysis buffer are used as negative controls. Cells treated with DMSO (0.1%) are treated as vehicle control. The reaction mixtures are incubated for 1 h at 37°C. The AMC liberated from the substrates is measured using spectrofluorometer of Victor 2 plate reader with an excitation wavelength of 380 nm and an emission wavelength of 430 nm.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: Chloroform : Soluble
DMSO : 59 mg/mL(179.7 mM)


关键字: Columbianadin | Inhibitor | inhibit | Apoptosis

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