盐酸乌苯美司 T3529
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
2 mg | 65391-42-6 | ¥223.00 | 询底价 |
500 mg | 65391-42-6 | ¥6,420.00 | 询底价 |
25 mg | 65391-42-6 | ¥987.00 | 询底价 |
10 mg | 65391-42-6 | ¥578.00 | 询底价 |
200 mg | 65391-42-6 | ¥3,930.00 | 询底价 |
100 mg | 65391-42-6 | ¥2,690.00 | 询底价 |
5 mg | 65391-42-6 | ¥397.00 | 询底价 |
1 mL | 65391-42-6 | ¥397.00 | 询底价 |
50 mg | 65391-42-6 | ¥1,660.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Bestatin hydrochloride
描述: Bestatin hydrochloride (Ubenimex hydrochloride) 是CD13(Aminopeptidase N)/APN 和leukotriene A4 hydrolase 抑制剂,可研究癌症。
细胞实验: Growing cells (1×106 to 2×106 cells/mL) are diluted to 1.0×103 cells/mL and transferred (3 mL) into a well in a 12-well multiwell plate (2.5-cm diameter/well). Cells are treated with 0, 10, 50, 100, 300, or 600 μM Bestatin and allowed to grow at 21°C shaking at 180 rpm for 48 h. A hemocytometer is used to measure cell density after 0, 24, and 48 h.
激酶实验: Cells are harvested, washed, and lysed in NP-40 lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 0.5% NP-40). Total cell protein is quantified using the Bradford assay and 1-mg/mL protein aliquots are made. Ten microliters of total cell protein is mixed with 290 μL of substrate solution (0.1 mg/mL dithiothreitol [DTT], 0.1 mg/mL albumin, and 1 mM alanine-β-naphthylamide). Fluorometric measurements (340 nm excitation, 400 nm emission) are made after 15 and 30 min. The slope of the line between the 15- and 30-min measurements is used to represent aminopeptidase activity. Total cell protein is preincubated with bestatin, amastatin, puromycin, EDTA, and/or ZnCl2 for 20 min before the fluorometric aminopeptidase assay.
体外活性: Bestatin, also known as Ubenimex (INN), is a competitive protease inhibitor. It is an inhibitor of aminopeptidase B, leukotriene A4 hydrolase, aminopeptidase N. It is being studied for use in the treatment of acute myelocytic leukemia. Bestatin can be administered, with low toxicity, to cultured cells, intact animals and humans. It has become a useful tool in elucidating the physiological role of some mammalian exopeptidases in the regulation of the immune system, in the growth of tumors and their invasion of surrounding tissues, and in the degradation of cellular proteins.
体内活性: Bestatin is an active anti-angiogenic agent that may inhibit tumor angiogenesis in vivo and tube-like formation of endothelial cells in vitro through its inhibition of APN/CD13 activity. Oral administration of Bestatin (100-200 mg/kg/day) is found to significantly inhibit the melanoma cell-induced angiogenesis in a mouse dorsal air sac assay.
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 50 mg/mL (144.99 mM)
H2O : 20 mg/mL (58.00 mM)
关键字: Bestatin Hydrochloride | Aminopeptidase | Bestatin | Inhibitor | Antibiotic | Bacterial | Bestatin hydrochloride | inhibit | Ubenimex Hydrochloride | Ubenimex
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途