化合物iCRT3 T4302

Product Introduction
Bioactivity


英文名: iCRT3

描述: iCRT3 是一种Wnt 和 β-连环蛋白应答转录抑制剂。

细胞实验: Cells are seeded into 96-well plates to a density of 5×103 cells per well and incubated in the culture medium with iCRT3 for an additional 48 h. Cell viability and cell apoptosis assays are carried out using a Cell Counting kit-8 and a Caspase-Glo 3/7 assay kit according to the manufacturer's instructions, respectively.

激酶实验: In the determination of full-length baculovirus expressed Syk kinase activity, the reaction system was 25 μL containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerophosphate, 2 mM DTT, 0.1 mM Na3VO4, 10 mM MgCl2, 0.5 μM Promega PTK Biotin Peptide Substrate 1, 0.01% casein, 1, 0.01% Triton X-100, 0.25% Glycerol, and 40 mM ATP (Km for ATP). After incubation for 60 minutes at room temperature, the reaction was stopped by adding 30 mM EDTA (30 μL of SA-APC and 4 nM PT-66 antibody). After measuring the plate, the IC50 value of the test compound was calculated using a 4-parameter linear regression algorithm.

动物实验: NOD-SCID BALB/c mice inoculated A172 cells are treated with iCRT3 (5 mg/kg) diluted in PBS i.p. triweekly.

体外活性: 使用iCRT3进行处理，剂量分别为12.5、25、50和75 μM，可以分别降低TNF-α水平的概率为14.7%、18.5%、44.9%和61.3%。

体内活性: 在10mg/kg iCRT3处理的小鼠中，IL-6水平较低。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (126.73 mM)


关键字: Inhibitor | Wnt | Apoptosis | inhibit | iCRT-3 | iCRT3 | iCRT 3

相关产品: GDC-0623 | Y-27632 | Etidronic acid | BIO-acetoxime | Notopterol | Carvacrol | Entinostat | Diallyl Trisulfide | TAS6417 | BGT226 maleate

相关库: Anti-Prostate Cancer Compound Library | Wnt/Hedgehog/Notch Compound Library | Anti-Cancer Metabolism Compound Library | Neural Regeneration Compound Library | Inhibitor Library | Anti-Colorectal Cancer Compound Library | Cytoskeletal Signaling Pathway Compound Library | Anti-Obesity Compound Library | Apoptosis Compound Library | Stem Cell Differentiation Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途