化合物USP7/USP47 inhibitor T4338

Product Introduction
Bioactivity


英文名: USP7/USP47 inhibitor

描述: USP7/USP47 inhibitor (USP7/47 inhibitor-1) 是一种选择性泛素特异性蛋白酶 7/47 抑制剂，EC50值分别为 0.42 μM 和 1.0 μM。

激酶实验: The cloning, expression and purification of USP21 from BL21 (DE3) bacteria are performed using standard molecular biology techniques. USP2, USP5, USP7, USP8, USP28, USP47, Ub-PLA2 (Ub-CHOP) and Ub-EKL (Ub-CHOP2) are generated. Caspase 3 and the caspase 3 substrate DEVD-Rh110 are used. Deubiquitylating enzyme, cathepsin B and 20S proteasome chymotrypsin like protease activities are measured. Caspase 3 activity is determined using a similar protocol. Briefly, dose ranges of compound (including USP7/USP47 inhibitor) are incubated with caspase 3 for 30 minutes before the addition of DEVD-Rh110 and reading on a fluorometric plate reader using excitation and emission maxima of 485 nm and 531 nm respectively. The final concentrations of caspase 3 and DEVD-Rh110 are 2 nM and 100 nM respectively

体外活性: USP7/USP47 inhibitor does not inhibit caspase 3, calpain 1, 20S proteasome, and a panel of representative USPs (USP2, USP5, USP8, USP21, and USP28; EC50 > 31.6 μM). USP7/USP47 inhibitor inhibits the growth of HCT-116 cells with an EC50 of 7.6 μM.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 44 mg/mL


关键字: USP-7/USP47 inhibitor | Deubiquitinase | inhibit | USP7/USP47 inhibitor | Inhibitor | DUBs

相关产品: ML364 | KSQ-4279 | DUB-IN-1 | GRL0617 | IU1-248 | USP8-IN-3 | Degrasyn | USP7-IN-1 | ML-323 | DUB-IN-3

相关库: Anti-Aging Compound Library | HIF-1 Signaling Pathway Compound Library | Cell Cycle Compound Library | Anti-Cancer Compound Library | Bioactive Compounds Library Max | Ubiquitination Compound Library | Inhibitor Library | DNA Damage & Repair Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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