化合物CeMMEC1 T4345

Product Introduction
Bioactivity


英文名: CeMMEC1

描述: CeMMEC1 是一种 BRD4 抑制剂，对 TAF1 有很好的亲和力，IC50为0.9 μM。

细胞实验: REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours.

激酶实验: TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves

体外活性: CeMMEC1 exhibits high affinity activity for the bromodomains of CREBBP, EP300, BRD9 and the second bromodomain of TAF1.

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 5.63 mg/mL (16.72 mM)


关键字: Epigenetic Reader Domain | CeMMEC-1 | Inhibitor | DNA/RNA Synthesis | inhibit | CeMMEC1 | CeMMEC 1

相关产品: Inobrodib | GSK9311 | PROTAC BRD4 Degrader-21 | dBET1 | CBP/p300-IN-21 | BAZ1A-IN-1 | Crebinostat | VTP50469 | GNE-207 | ACBI1

相关库: Anti-Aging Compound Library | Anti-Metabolism Disease Compound Library | Bioactive Compounds Library Max | Kinase Inhibitor Library | Inhibitor Library | Epigenetics Compound Library | DNA Damage & Repair Compound Library | Histone Modification Compound Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途