化合物ERK5-IN-1 T5184
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
10 mg | 1234479-76-5 | ¥1,730.00 | 询底价 |
5 mg | 1234479-76-5 | ¥1,230.00 | 询底价 |
100 mg | 1234479-76-5 | ¥6,630.00 | 询底价 |
1 mg | 1234479-76-5 | ¥495.00 | 询底价 |
50 mg | 1234479-76-5 | ¥4,650.00 | 询底价 |
1 mL | 1234479-76-5 | ¥1,260.00 | 询底价 |
25 mg | 1234479-76-5 | ¥3,180.00 | 询底价 |
Product Introduction
Bioactivity
英文名: ERK5-IN-1
描述: ERK5-IN-1 是 ERK5抑制剂,IC50为 87 nM。它也抑制 LRRK2[G2019S],IC50为 26 nM。
细胞实验: HeLa cells were serum starved overnight followed by treatment with inhibitors for 1 h. Cells were then stimulated with EGF (20 ng/mL) for 17 min and harvested in RIPA buffer (1× PBS, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 0.1 mg/ml PMSF and 1 mM sodium orthovanadate). Proteins from total cell lysates were resolved by 6% sodium dodecyl sulfate (SDS)-poly-acrylamide gel electrophoresis (PAGE), transferred to nitrocellulose membrane, blocked in 5% nonfat milk, and blotted with anti-ERK5 antibody.
激酶实验: Kinase activity was determined in an assay volume of 40 μL in kinase buffer (50 mM Tris–HCl, pH 7.5, 0.1 mM EGTA, 1 mM 2-mercaptoethanol) containing 200 ng of pure active ERK5 and the indicated amount of inhibitor. Reaction started by adding 10 mM magnesium acetate, and 50 μM [γ-32P]-ATP (500 cpm/pmol) and 250 μM PIMtide (ARKKRRHPSGPPTA) as substrates. Assays were carried out for 20 min at 30 °C, terminated by applying the reaction mixture onto p81 paper and the incorporated radioactivity measured as described previously.
体外活性: ERK5-IN-1能够抑制HeLa细胞中由EGF刺激的ERK5自磷酸化(EC50:0.19 μM)。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 25 mg/mL (54.4 mM)
关键字: ERK5-IN-1 | ERK5 IN 1 | ERK | Inhibitor | ERK-5-IN-1 | Extracellular signal regulated kinases | ERK5IN1 | inhibit
相关产品: BAY885 | Rineterkib | Pinusolide | A-674563 | EF24 | ERK1/2 inhibitor 9 | 7,8-Didehydrocimigenol | Nitidine chloride | Griffipavixanthone | Desoxo-narchinol A
相关库: HIF-1 Signaling Pathway Compound Library | Anti-Prostate Cancer Compound Library | Bioactive Compounds Library Max | Anti-Ovarian Cancer Compound Library | Inhibitor Library | Kinase Inhibitor Library | Anti-Colorectal Cancer Compound Library | Anti-Pancreatic Cancer Compound Library | MAPK Inhibitor Library | Bioactive Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途