特丁基对苯二酚 T5364
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mL | 1948-33-0 | ¥365.00 | 询底价 |
1 g | 1948-33-0 | ¥415.00 | 询底价 |
500 mg | 1948-33-0 | ¥331.00 | 询底价 |
Product Introduction
Bioactivity
英文名: TBHQ
描述: TBHQ (tert-Butylhydroquinone) 是一种抗氧化剂,可通过对氧化还原敏感的转录因子 Nrf2 诱导抗氧化反应。它也是一种ERK 激活剂,逆转 Dehydrocorydaline 对黑素瘤细胞增殖的抑制作用。
细胞实验: Jurkat T cells were cultured in 96-well plates (1×10^5 cells/well) and treated as described, and cell supernatants were harvested 24h after activation with anti-CD3/anti-CD28. IL-2 was quantified from cell supernatants by the use of a commercially available kit per the manufacturer's protocol. Relative light intensity was quantified at 450nm by Bio-Tek μQuant microplate reader. The IL-2 concentrations of each sample were then calculated using a line ar standard curve on Microsoft Excel [2].
动物实验: TBHQ powder was directly mixed with the animal food powder at a ratio of 1% (w/w) as described previously. The efficacy of this protocol for TBHQ treatment in vivo has also been validated by other groups. TBHQ treatment was continued for 4 weeks after the TAC surgery [3].
体外活性: HeLa cells treated with tBHQ showed preferential oxidation of mitochondrial thioredoxin-2 (Trx2), while cellular glutathione and cytosolic thioredoxin-1 were not affected. With tBHQ treatments, Trx2 overexpression suppressed Nrf2 accumulation and activity [1]. Pretreatment of Jurkat T cells with tBHQ, prior to activation with anti-CD3/anti-CD28, diminished the production of interleukin-2 (IL-2) at both the transcript and protein levels. Similarly, the expression of CD25 also diminished, albeit to a lesser degree than IL-2, after pretreatment with tBHQ [2].
体内活性: TBHQ treatment prevented left ventricular dilatation and cardiac dysfunction induced by TAC and decreased the prevalence of myocardial apoptosis. TBHQ-induced Akt activation was accompanied by increased phosphorylation of Bad, glycogen synthase kinase-3β (GSK-3β) and mammalian target of rapamycin (mTOR). Blockade of the Akt pathway in vivo accelerated cardiac dysfunction, and abrogated the protective effects of TBHQ. TBHQ also reduced the reactive aldehyde production and protein carbonylation in stressed myocardium [3].
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 50 mg/mL (300.81 mM)
关键字: Ferroptosis | Keap1-Nrf2 | Apoptosis | ERK | inhibit | Autophagy | TBHQ | Extracellular signal regulated kinases | Inhibitor
相关产品: Nutlin-3a | BTT-3033 | LYG-202 | PU02 | DEL-22379 | Romidepsin | Etoposide | Madecassoside | Nitroaspirin | Ganoderenic acid D
相关库: Anti-Aging Compound Library | Anti-Neurodegenerative Disease Compound Library | Anti-Prostate Cancer Compound Library | Oxidation-Reduction Compound Library | Food Additive Library | Ferroptosis Compound Library | Kinase Inhibitor Library | Anti-Obesity Compound Library | Stem Cell Differentiation Compound Library | Anti-Alzheimer's Disease Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途