新藤黄酸 T5724
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
1 mL | 93772-31-7 | ¥990.00 | 询底价 |
25 mg | 93772-31-7 | ¥1,480.00 | 询底价 |
50 mg | 93772-31-7 | ¥2,230.00 | 询底价 |
10 mg | 93772-31-7 | ¥883.00 | 询底价 |
100 mg | 93772-31-7 | ¥3,330.00 | 询底价 |
1 mg | 93772-31-7 | ¥272.00 | 询底价 |
5 mg | 93772-31-7 | ¥619.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Neogambogic acid
描述: Neogambogic acid 是藤黄的有效成分,可诱导细胞凋亡并具有抗癌作用。它有效抑制耐甲氧西林的金黄色葡萄球菌。
细胞实验: MCF-7 cells (5* 10^4 ) were seeded into 96-well plates.Four hours later, 10 ul NGA in DMSO was added into the wells at various concentrations (0.5-24 ug/ml) and 0.1% DMSO was set as a negative control.?After 72 h, 50 ul MTT was added and cells were incubated for another 4 h. Then, media was removed and 150 ul DMSO was added and the plates were placed on a shaking table at 150 rpm for 10 min. Optical density (OD) was measured at 490 nm.?The experiment was repeated thrice and the rate of cell inhibition was calculated using the following formula: inhibition rate [1-(OD test/ OD negative control)]*100%[1].
动物实验: Primary mouse BMMs were cultured with increasing concentrations of NGA.?Real-time polymerase chain reaction was used to study the expression of mRNAs corresponding to gene products specific to receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation, including tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), cathepsin K (CTSK), and nuclear factor of activated T cells c1 (NFATc1).?A cell counting kit-8 assay was used to evaluate cell proliferation.?Western blotting and confocal immunofluorescence microscopy were used to investigate the signaling pathways.?A bone resorption model was used to quantify bone resorption[2].
体外活性: Neo-gambogic acid(NGA) significantly reduced the proliferation of MCF-7 cells in a dose-dependent manner. NGA could increase the expression of the apoptosis-related proteins FasL, caspase-3, caspase-8, caspase-9, and Bax and decrease the expression of anti-apoptotic protein Bcl-2 accompanied by the mitochondrial transmembrane damage. The antiproliferative effect of NGA on MCF-7 cells is due to the G(0)/G(1) arrest, increased apoptosis and activation of Fas/FasL and cytochrome C pathway[1].
体内活性: Neo-gambogic acid(NGA) dose of ≤0.4 μg/ml had no significant effect on the proliferation of mouse BMMs in vitro (P>0.05);?concentrations of between 0.1-0.4 μg/ml significantly inhibited RANKL-induced osteoclastogenesis (P<0.01) in a dose-dependent manner.?Compared with the control group, NGA significantly reduced RANKL-induced bone resorption in vitro (P <0.01), and downregulated the expression of osteoclast-related mRNAs of TRAP, CTR, CTSK, and NFATc1.?NGA suppressed the activation of JNK but not the p38 signaling pathway and significantly reduced NF-κB p65 phosphorylation and the nuclear transport of NF-κB molecules, which inhibited NFATc1 expression.?CONCLUSIONS NGA suppressed RANKL-induced osteoclastogenesis by inhibiting the JNK and NF-κB pathways in mouse BMMs in vitro and reduced osteoclastic bone resorption[2].
存储条件: keep away from direct sunlightPowder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 6.47 mg/mL (10 mM)
关键字: cancer | MRSA | Apoptosis | arrest | breast | MCF-7 | Bacterial | Inhibitor | Neogambogic acid | inhibit | tummor | cells | proliferation
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相关库: Anti-Tumor Natural Product Library | Covalent Natural Product Library | Bioactive Compounds Library Max | Anti-Cancer Active Compound Library | Natural Product Library for HTS | Covalent Inhibitor Library | NO PAINS Compound Library | Anti-Infection Compound Library | Anti-infective Natural Product Library | Bioactive Compound Library
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