化合物Z-DEVD-FMK T6005

Product Introduction
Bioactivity


英文名: Z-DEVD-FMK

描述: Z-DEVD-FMK (Caspase-3 Inhibitor) 是一种选择性、不可逆的 Caspase-3 抑制剂，IC50为 18 μM。 它也抑制 caspase-6、caspase-7、caspase-8 和 caspase-10。

细胞实验: N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT (3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl tetrazolium bromide) assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/ml MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader.(Only for Reference)

激酶实验: Caspase activity assay : Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade

体内活性: Z-DEVD-FMK 在伤害前后显著减少了创伤后细胞凋亡，并显著提高了神经功能恢复。[2]

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 50 mg/mL (74.78 mM)
5% DMSO+95% Saline : 4.65 mg/mL (6.95 mM, suspension)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)


关键字: ZDEVDFMK | Inhibitor | Z DEVD FMK | Caspase | inhibit | Z-DEVD-FMK

相关产品: 3-O-trans-p-Coumaroyltormentic acid | Myricanone | Wedelolactone | Quinovic acid | Sageone | Cystamine | Caspase-3 activator 2 | Santamarine | Nivalenol | Zingiberen newsaponin

相关库: Anti-Neurodegenerative Disease Compound Library | Anti-COVID-19 Compound Library | Pyroptosis Compound Library | Fluorochemical Library | Inhibitor Library | Highly Selective Inhibitor Library | Apoptosis Compound Library | Protease Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library

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注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途