阿洛司他丁 T6040
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
25 mg | 88321-09-9 | ¥2,530.00 | 询底价 |
500 mg | 88321-09-9 | ¥10,800.00 | 询底价 |
10 mg | 88321-09-9 | ¥1,470.00 | 询底价 |
1 mg | 88321-09-9 | ¥313.00 | 询底价 |
5 mg | 88321-09-9 | ¥842.00 | 询底价 |
2 mg | 88321-09-9 | ¥476.00 | 询底价 |
1 mL | 88321-09-9 | ¥877.00 | 询底价 |
100 mg | 88321-09-9 | ¥5,290.00 | 询底价 |
50 mg | 88321-09-9 | ¥3,730.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Aloxistatin
描述: Aloxistatin (E64d) 是一种不可逆的、可透过膜的溶酶体和细胞溶质半胱氨酸蛋白酶抑制剂,能够抑制完整血小板中的钙蛋白酶活性。它是一种半胱氨酸蛋白酶抑制剂,具有血小板聚集抑制活性。
细胞实验: Aloxistatin (E64d) is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.1 %) before use[3]. Cell proliferation and apoptosis are assessed by staining for a proliferation marker, Ki67, or an apoptotic marker, cleaved caspase 3, following the protocol described above for the polarity markers. MCF10 variants are grown in 3D rBM overlay cultures for 4 days and are treated with 0.1 % DMSO, 5 μM CA074Me or 5 μM Aloxistatin. The percentage of structures that are positive for Ki67 or cleaved caspase 3 is determined by counting a total of 100 structures on two separate coverslips with a Zeiss Axiophot epifluorescent microscope. Structures are considered Ki67 positive if they contained at least one cell staining for Ki67. Structures are considered to be caspase 3 positive if they contained at least one cell that is positive for cleaved caspase 3 and the positive cell(s) is not localized in the center of a developing lumen[3].
激酶实验: The CTLs and NK cells (0.8×106/mL) are treated with the inhibitors L1 (10-20 μM) or Aloxistatin (20-30 μM) for 24 hr at 37°C in 24-well plates. Cells are then used in 51Cr-release assays or are lysed to examine perforin in Western blots. The inhibitor is also added at the same concentration during the 4 hr reactions in some 51Cr-release assays, as indicated. Cell lysates are prepared using NP-40 lysis buffer (25 mM HEPES, 250 mM NaCl, 2.5 mM ethylenediaminetetraacetic acid, 0.1% volume/volume Nonidet P-40) and total protein concentration is determined using the Bradford assay. Equal amounts of protein are loaded and resolved on 8% SDS-PAGE gels. Human or mouse perforin is detected using the appropriate antibodies as indicated. Anti-actin antibody is used as a loading control[2].
体外活性: Aloxistatin can enter the intact platelet and inhibit proteolysis by inhibiting calpain. [2] Aloxistatin blunts Parathyroid hormone (PTH)-induced cell proliferation and inhibits differentiation osteoblasts in vitro. [3]
体内活性: Aloxistatin (100 mg/kg, p.o.) strongly inhibits the cathepsin B&L activities in the skeletal muscle, heart and liver of hamsters. [1] In spinal cord injury (SCI) rats, Aloxistatin provides neuroprotection in SCI lesion and penumbra. [4] Aloxistatin reduces brain amyloid-β and improves memory deficits in Alzheimer's disease animal models by inhibiting cathepsin B activity. [5]
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: DMSO : 55 mg/mL (160.62 mM)
关键字: Cathepsin | SARS-CoV | Inhibitor | inhibit | Aloxistatin | SARS coronavirus
相关产品: MAT-POS-e194df51-1 | HCoV-OC43-IN-1 | SARS-CoV-2-IN-65 | Ledipasvir | ML188 | Setrobuvir | Chloroquine | Molnupiravir | SIMR3030 | Imatinib
相关库: Anti-Aging Compound Library | ReFRAME Related Library | Bioactive Compounds Library Max | Endoplasmic Reticulum Stress Compound Library | Inhibitor Library | Highly Selective Inhibitor Library | Target-Focused Phenotypic Screening Library | Protease Inhibitor Library | Covalent Inhibitor Library | Anti-Viral Compound Library
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途