化合物 SK1-?I hydrochloride T60798

Product Introduction
Bioactivity


英文名: SK1- I hydrochloride

描述: SK1-I hydrochloride (BML-258 hydrochloride) 是鞘氨醇类似物，能够增强细胞自噬并显示抗癌活性。SK1-I hydrochloride 是同工酶特异性SPHK1竞争抑制剂 (Ki = 10 μM)。

体外活性: SK1-I hydrochloride (0-10 μM; 24 hours) attenuates cancer cell growth and survival in a TP53-dependent manner in HCT116 cells and HCT116 cells bearing TP53 null cancer [2]. SK1-I hydrochloride (0-20 μM; 12 hours) induces more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53, leading to a hallmark of apoptosis [2]. Cell Viability Assay [2] Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer Concentration: 0 μM, 2.5 μM, 5 μM, 7.5 μM, 10 μM Incubation Time: 24 hours Result: Decreased cancer cell growth and survival. Western Blot Analysis [2] Cell Line: HCT116 cells and HCT116 cells bearing TP53 null cancer Concentration: 0 μM, 5 μM, 10 μM, 20 μM Incubation Time: 12 hours Result: Induced more CASP3 cleavage in HCT116 cells, compared to HCT116 cells lacking TP53.

体内活性: Pre-treatment with SK1-I hydrochloride (BML-258 hydrochloride; intraperitoneal (i.p.) injection; once; 24 hours prior to baseline mean arterial blood pressure (MAP) measurement; 75 mg/kg) before anandamide (i.v. injection; two doses; 1 and 10 mg/kg) significantly decreases the hypotensive response [3]. Animal Model: Male C57BL/6 mice (24 ± 3.5 g) [3] Dosage: 75 mg/kg Administration: Intraperitoneal (i.p.) injection; once; 24 hours prior to baseline MAP measurement Result: Significantly lowered baseline mean arterial blood pressure (MAP).

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

化合物 SK1-?I hydrochloride T60798信息由TargetMol中国为您提供，如您想了解更多关于化合物 SK1-?I hydrochloride T60798报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途