化合物AEBSF hydrochloride T6370

Product Introduction
Bioactivity


英文名: AEBSF hydrochloride

描述: AEBSF hydrochloride (Pefabloc SC) 是一种丝氨酸蛋白酶的不可逆抑制剂。

细胞实验: The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.

体外活性: 在蟑螂诱发的小鼠过敏模型中,AEBSF能够减少其呼吸道反应和潜在的炎症.在小鼠中注射致死量的弓形虫,每天腹腔注射76.8 mg/kg AEBSF,能够延长其生存期.

体内活性: AEBSF是一种丝氨酸蛋白酶抑制剂,它能够抑制巨噬细胞裂解白血病细胞,并且不会抑制巨噬细胞分泌TNF-α和IL-1β。在5种不同人细胞系中,AEBSF通过抑制神经和非神经中的β分泌,从而抑制组成Aβ的产物。AEBSF能够扰乱子宫内膜中囊胚的生长,还能够改变蛋白分泌机制从而抑制在人脐静脉内皮细胞中HeLa细胞的粘附。

存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: H2O : 24 mg/mL (100 mM)
DMSO : 60 mg/mL (250.32 mM)


关键字: Thrombin | Influenza Virus | AEBSF hydrochloride | AEBSF | Ser/Thr Protease | Threonine proteases | inhibit | AEBSF Hydrochloride | Serine endopeptidases | Serine proteases | Inhibitor

相关产品: Heparin | Imitrodast | Odiparcil | RS-601 | Thrombin receptor peptide ligand | NFPS | Eltrombopag Olamine | Flovagatran | Melagatran | Dabigatran ethyl ester hydrochloride

相关库: Anti-Aging Compound Library | Anti-Metabolism Disease Compound Library | Bioactive Compounds Library Max | Fluorochemical Library | Inhibitor Library | Highly Selective Inhibitor Library | Anti-Viral Compound Library | Covalent Inhibitor Library | NO PAINS Compound Library | Bioactive Compound Library

化合物AEBSF hydrochloride T6370信息由TargetMol中国为您提供，如您想了解更多关于化合物AEBSF hydrochloride T6370报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途