埃博霉素 A T6490
品牌 | 厂商性质 | 产地 | 货期 |
---|---|---|---|
TargetMol | 生产商 | 美国 | 现货 |
性能特点
生化试剂,可用于动物细胞实验
规格 | CAS号 | 价格 | 操作 |
---|---|---|---|
50 mg | 152044-53-6 | ¥3,660.00 | 询底价 |
5 mg | 152044-53-6 | ¥747.00 | 询底价 |
200 mg | 152044-53-6 | ¥7,480.00 | 询底价 |
2 mg | 152044-53-6 | ¥498.00 | 询底价 |
10 mg | 152044-53-6 | ¥1,180.00 | 询底价 |
1 mL | 152044-53-6 | ¥813.00 | 询底价 |
100 mg | 152044-53-6 | ¥5,490.00 | 询底价 |
500 mg | 152044-53-6 | ¥10,900.00 | 询底价 |
1 mg | 152044-53-6 | ¥372.00 | 询底价 |
25 mg | 152044-53-6 | ¥2,390.00 | 询底价 |
Product Introduction
Bioactivity
英文名: Epothilone A
描述: Epothilone A (Epo A) 是紫杉醇和管蛋白聚合物结合的一种竞争性抑制剂,其Ki 值为 0.6-1.4 μM。
细胞实验: Cell lines: KB3-1,KBV-1,Hela,and Hs578T cells. Concentrations: 0–2 μM. Method: cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips.After 24 hours,cells are treated with Epothilone A and are scored at regular intervals.For the cytotoxicity analysis,cells are counted and scored as trypan blue positive or negative.
激酶实验: Tubulin polymerization assay: Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone A is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope.
体内活性: Epothilone A诱导微管蛋白聚合, 导致细胞周期在G2-M期停滞, 具有细胞毒性,也诱导细胞凋亡。Epothilone A有效抑制HCT116细胞增殖,IC50为4.4 nM。Epothilone A 对KB3-1, KBV-1, Hela和 Hs578T细胞具有细胞毒性,IC50为13 nM到160 nM。Epothilone A与紫杉醇竞争性与微管结合,IC50为2.3 μM。
存储条件: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
溶解度: H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 92 mg/mL (186.4 mM)
DMSO : 92 mg/mL (186.4 mM)
关键字: Inhibitor | inhibit | Apoptosis | Epothilone A | Microtubule/Tubulin
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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途