N'-硝基-L-精氨酸甲酯盐酸盐 T6570

Product Introduction
Bioactivity


英文名: L-NAME hydrochloride

描述: L-NAME hydrochloride (L-NAME HCl) 是NOS 抑制剂(IC50:70 μM)。

细胞实验: rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. (Only for Reference)

激酶实验: Enzyme Assay: The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated.

体内活性: L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2]

存储条件: store at low temperaturePowder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度: DMSO : 50 mg/mL (185.4 mM)
H2O : 27 mg/mL (100 mM)


关键字: inhibit | NG-Nitroarginine methyl ester Hydrochloride | Inhibitor | NO Synthase | NOS | L-NAME | Nitric oxide synthases | L-NAME Hydrochloride | LNAME hydrochloride | L NAME hydrochloride | NG-Nitroarginine methyl ester

相关产品: Isodunnianol | FK-330 | MEG hemisulfate | Ermanin | Latifolin | NOS1-IN-1 | Flavoglaucin | Catalposide | Cyclomorusin | Demethyleneberberine

相关库: Anti-Aging Compound Library | Drug Repurposing Compound Library | Clinical Compound Library | Bioactive Compounds Library Max | Inhibitor Library | Nonsteroidal Anti-Inflammatory Compound Library | NO PAINS Compound Library | Bioactive Compound Library

N'-硝基-L-精氨酸甲酯盐酸盐 T6570信息由TargetMol中国为您提供，如您想了解更多关于N'-硝基-L-精氨酸甲酯盐酸盐 T6570报价、型号、参数等信息，欢迎来电或留言咨询。

注：该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案，不可用于临床诊断或治疗等相关用途